Differential effect of Pistacia vera extracts on experimental atherosclerosis in the rabbit animal model: an experimental study

Lipids in Health and Disease

Research

Efstratios Patsouris¹  George Agrogiannis¹  Achilles Chatziioannou²  Maria Halabalaki³  Katerina Georgopoulou³  Prokopios Magiatis³  Leandros A Skaltsounis³  Apostolos Papalois⁴  Nektaria Tsantila⁵  Dimitrios Iliopoulos⁶  Theodore Karatzas⁷  Ismene A Dontas⁸  Katerina A Marinou⁹ 

[1] 1st Department of Pathology, School of Medicine, University of Athens, Greece;1st Department of Radiology, Aretaieion University Hospital, School of Medicine, University of Athens, Greece;Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, University of Athens, Greece;Experimental - Research Center ELPEN Pharma, Pikermi, Greece;Laboratory of Biochemistry, Department of Chemistry, University of Athens, Greece;Laboratory of Experimental Surgery and Surgical Research "Christeas Hall", School of Medicine, University of Athens, Greece;Laboratory of Experimental Surgery and Surgical Research "Christeas Hall", School of Medicine, University of Athens, Greece;2nd Department of Propedeutic Surgery, Laiko Hospital, School of Medicine, University of Athens, Greece;Laboratory of Experimental Surgery and Surgical Research "Christeas Hall", School of Medicine, University of Athens, Greece;Laboratory for Research of the Musculoskeletal System, School of Medicine, University of Athens, Greece;Laboratory of Experimental Surgery and Surgical Research "Christeas Hall", School of Medicine, University of Athens, Greece;Ministry of Rural Development and Food, Centre of Athens Veterinary Institutes, Agia Paraskevi, Attiki, Greece;

关键词: Catechin;    Methanolic Extract;    Atherosclerotic Lesion;    Protocatechuic Acid;    Hydroxytyrosol;   

DOI  :  10.1186/1476-511X-9-73

 received in 2010-03-29, accepted in 2010-07-16,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundLipid-enriched diets and oxidative stress are risk factors for the development of atherosclerosis. The effects of the methanolic (ME) and cyclohexane (CHE) extracts of the Pistacia vera nut, often included in the Mediterranean diet, were studied in the rabbit model of atherosclerosis.Methods and resultsTwenty-four New Zealand White rabbits received atherogenic diet (Control Group), supplemented with ME (Group ME) or CHE (Group CHE) for 3 months. Previously, a GC-MS and a UHPLC LC-DAD-ESI(-)-HRMS/MS method were developed to investigate the extracts' chemical profiles. Blood samples at baseline and monthly determined lipid profile, lipid peroxidation and liver function. The aorta, myocardium and liver were examined histologically at 3 months.Groups ME and CHE had significantly higher HDL- and non-significantly lower LDL-cholesterol median % changes from baseline than the Control Group. Triacylglycerol was significantly higher in Group CHE vs. Control. MDA values were significantly lower in Group ME vs. Control and CHE. ALT and AST were significantly higher in Group CHE vs. Control. γ-GT was lower in Group ME vs. Control. Aortic intimal thickness was significantly less in Groups ME and CHE vs. Control; Group ME atherosclerotic lesions were significantly less extensive vs. Groups Control and CHE. Only Group CHE had significant liver fatty infiltration.ConclusionsDuring short-term administration concomitantly with atherogenic diet, both P. vera extracts were beneficial on HDL-, LDL-cholesterol and aortic intimal thickness. The ME additionally presented an antioxidant effect and significant decrease of aortic surface lesions. These results indicate that P. vera dietary inclusion, in particular its ME, is potentially beneficial in atherosclerosis management.

【 授权许可】

Unknown   
© Marinou et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.