Molecular changes induced by the curcumin analogue D6 in human melanoma cells

Molecular Cancer

Research

Carla Rozzo¹  Marina Pisano¹  Antonio Corrias¹  Giuseppe Palmieri¹  Davide Fabbri¹  Sara Cossu¹  Maria Antonietta Dettori¹  Valentina Nieddu¹  Emanuela Azara¹  Ilaria Sassu¹  Cristina Fraumene²  Tiziana Cubeddu²  Grazia Galleri²  Manuela Fanciulli² 

[1] Biomolecular Chemistry Institute, National Research Council of Italy, Traversa La Crucca, 3, 07100, Sassari, ITALY;Porto Conte Ricerche srl., Località Tramariglio, Alghero, (SS), ITALY;

关键词: Curcumin;    Melanoma Cell;    Fold Change;    Ingenuity Pathway Analysis;    Noxa;   

DOI  :  10.1186/1476-4598-12-37

 received in 2012-11-30, accepted in 2013-04-26,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundIn a previous report, we described the in vitro and in vivo antiproliferative and proapoptotic activity of a hydroxylated biphenyl (D6), a structural analogue of curcumin, on malignant melanoma and neuroblastoma tumours. In this paper, we investigated the molecular changes induced by such a compound, underlying cell growth arrest and apoptosis in melanoma cells.ResultsTo shed light on the mechanisms of action of D6, we firstly demonstrated its quick cellular uptake and subsequent block of cell cycle in G2/M phase transition. A gene expression profile analysis of D6-treated melanoma cells and fibroblasts was then carried out on high density microarrays, to assess gene expression changes induced by this compound. The expression profile study evidenced both an induction of stress response pathways and a modulation of cell growth regulation mechanisms. In particular, our data suggest that the antiproliferative and proapoptotic activities of D6 in melanoma could be partially driven by up-regulation of the p53 signalling pathways as well as by down-regulation of the PI3K/Akt and NF-kB pathways. Modulation of gene expression due to D6 treatment was verified by western blot analysis for single proteins of interest, confirming the results from the gene expression profile analysis.ConclusionsOur findings contribute to the understanding of the mechanisms of action of D6, through a comprehensive description of the molecular changes induced by this compound at the gene expression level, in agreement with the previously reported anti-tumour effects on melanoma cells.

【 授权许可】

CC BY   
© Rozzo et al.; licensee BioMed Central Ltd. 2013