Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco

BMC Veterinary Research

Research Article

Jakob Zinsstag¹  Hind Yahyaoui-Azami²  Jaouad Berrada³  Mohammed Bouslikhane³  Soukaina Rami³  Hamid Aboukhassib⁴  Julia Feldmann⁵  Sonia Borrell⁵  Miriam Reinhard⁵  Sebastien Gagneux⁵ 

[1] Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland;University of Basel, Basel, Switzerland;Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland;University of Basel, Basel, Switzerland;Department of Pathology and Veterinary Public Health, Hassan II Agronomy and Veterinary Institute, Rabat, Morocco;Department of Pathology and Veterinary Public Health, Hassan II Agronomy and Veterinary Institute, Rabat, Morocco;Office Nationale de Sécurité Sanitaire des produits Alimentaires, Rabat, Morocco;University of Basel, Basel, Switzerland;Département de Biologie, Equipe Physiopathologies Nutritionnelles et Toxicologie, Faculté des Sciences UCD, El Jadida, Morocco;

关键词: Mycobacterium bovis;    Bovine tuberculosis;    Morocco;    Cattle;    Slaughterhouse;    Spoligotype;    PCR;   

DOI  :  10.1186/s12917-017-1165-6

 received in 2016-09-01, accepted in 2017-08-09,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundBovine tuberculosis (BTB) is caused by Mycobacterium bovis, which belongs to the Mycobacterium tuberculosis complex. Mycobacterium bovis have been described to be responsible of most cases of bovine tuberculosis. Although M. tuberculosis, M. africanum and non-complex mycobacteria were isolated from cattle.In Morocco, so far, no molecular studies were conducted to characterize the strains responsible of BTB. The present study aims to characterize M. bovis in Morocco.The present study was conducted in slaughterhouses in Rabat and El Jadida. Samples were collected from 327 slaughtered animals with visible lesions suggesting BTB.ResultsA total of 225 isolates yielded cultures, 95% (n = 215) of them were acid-fast (AF). Sixty eight per cent of the AF positive samples were confirmed as tuberculous mycobacteria (n = 147), 99% of these (n = 146) having RD9 and among the latter, 98% (n = 143) positive while 2% (n = 3) negative for RD4A total of 134 samples were analyzed by spoligotyping of which 14 were in cluster and with 41 different spoligotypes, ten of them were new patterns (23%). The most prevalent spoligotypes were SB0121, SB0265, and SB0120, and were already identified in many other countries, such as Algeria, Spain, Tunisia, the United States and Argentina.ConclusionThe shared borders between Algeria and Morocco, in addition to the previous importation of cattle from Europe and the US could explain the similarities found in M. bovis spoligotypes. On the other hand, the desert of Morocco could be considered as an efficient barrier preventing the introduction of BTB to Morocco from West Central and East Africa. Our findings suggest a low level endemic transmission of BTB similar to other African countries. However, more research is needed for further knowledge about the transmission patterns of BTB in Morocco.

【 授权许可】

CC BY   
© The Author(s). 2017