Neogenin expression is inversely associated with breast cancer grade in ex vivo

World Journal of Surgical Oncology

Research

Wanying Xing¹  Zheli Xu¹  Qiang Li²  Rangjuan Cao² 

[1] Department of Breast Surgery, China-Japan Union Hospital of Jilin University, 130033, Changchun, China;Department of Hand Surgery, China-Japan Union Hospital of Jilin University, 130033, Changchun, China;

关键词: Neogenin;    breast cancer;    histological grading;    predictive biomarkers;   

DOI  :  10.1186/1477-7819-12-352

 received in 2014-05-29, accepted in 2014-10-02,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundNeogenin is closely related to the human tumor suppressor gene deleted in colorectal cancer and plays a role in mammary morphogenesis. This study aimed to assess neogenin expression in breast cancer for any clinically significant association.MethodsA total of 54 breast cancer patients who underwent modified radical mastectomy were enrolled for immunohistochemical and quantitative real-time PCR analysis of neogenin expression in their cancerous breast tissues in comparison to matching distant non-cancerous tissues.ResultsThe data showed that the neogenin protein was either strongly or moderately expressed in the cytoplasm of the distant non-cancerous cells. In contrast, neogenin protein was either weakly or not expressed in the cytoplasm of 51/54 (94.4%) breast cancer cells, among which 13 breast cancer cases did not express neogenin protein at all (13/54, 24.1%). Similarly, levels of neogenin mRNA were significantly lower in breast cancer tissues than that of the matched distant non-cancerous tissues (51/54, 94.4%). Neogenin expression was inversely associated with breast cancer grade; that is, grade III breast cancer expressed much less neogenin than grade I-II (P < 0.05).ConclusionsThis study indicates that neogenin expression in breast cancer tissues is inversely associated with tumor grade.

【 授权许可】

Unknown   
© Xing et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.