Developmental patterns and characteristics of epicardial cell markers Tbx18 and Wt1 in murine embryonic heart

Journal of Biomedical Science

Research

Xiao-feng Ren¹  Jing Zhang²  Bin Zeng²  Feng Cao²  Xiao-yang Zhou² 

[1] College of Veterinary Medicine, Northeast Agricultural University, Harbin, Hei Longjiang, P. R, China;Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, P. R, China;

关键词: Cardiac progenitor;    Epicardial cells;    Tbx18;    Wt1;    Development;   

DOI  :  10.1186/1423-0127-18-67

 received in 2011-06-05, accepted in 2011-08-26,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundAlthough recent studies have highlighted the role of epicardial cells during cardiac development and regeneration, their cardiomyogenic potential is still controversial due to the question of lineage tracing of epicardial cells. The present study therefore aimed to examine the the expression of Tbx18 and Wt1 in embryonic heart and to identify whether Tbx18 and Wt1 themselves expressed in the cardiomyocyte.MethodsMouse embryonic hearts were collected at different stages for immunofluorescence costaining with either Tbx18 and the cardiac transcription factor Nkx2.5 or Wilms tumor 1 (Wt1) and Nkx2.5.ResultsTbx18 and Wt1, but not Nkx2.5, were expressed in the proepicardium and epicardium. Tbx18 was expressed in cells within the heart from E10.5 to at least E14.5; these Tbx18-expressing cells were Nkx2.5 positive, except for a few cells that were Nkx2.5 negative at E14.5. Wt1 was expressed in cells within the heart from E12.5 to at least E14.5, but these Wt1-expressing cells were Nkx2.5 negative.ConclusionThe data obtained in this study demonstrate that Tbx18 is expressed in the cardiomyocytes from E10.5 to at least E14.5, and Wt1 is expressed within the heart from E12.5 to at least E14.5, but not in the cardiomyocyte. These findings may provide new insights on the role of the epicardial cells in cardiac regeneration.

【 授权许可】

CC BY   
© Zeng et al; licensee BioMed Central Ltd. 2011