Developmental patterns and characteristics of epicardial cell markers Tbx18 and Wt1 in murine embryonic heart

Journal of Biomedical Science

Jing Zhang²  Xiao-yang Zhou²  Feng Cao²  Xiao-feng Ren¹  Bin Zeng² 

[1] College of Veterinary Medicine, Northeast Agricultural University, Harbin, Hei Longjiang, P. R. China;Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, P. R. China

关键词: Development;    Wt1;    Tbx18;    Epicardial cells;    Cardiac progenitor;   

Others  :  829430
DOI  :  10.1186/1423-0127-18-67

 received in 2011-06-05, accepted in 2011-08-26,  发布年份 2011

【 摘 要 】

Background

Although recent studies have highlighted the role of epicardial cells during cardiac development and regeneration, their cardiomyogenic potential is still controversial due to the question of lineage tracing of epicardial cells. The present study therefore aimed to examine the the expression of Tbx18 and Wt1 in embryonic heart and to identify whether Tbx18 and Wt1 themselves expressed in the cardiomyocyte.

Methods

Mouse embryonic hearts were collected at different stages for immunofluorescence costaining with either Tbx18 and the cardiac transcription factor Nkx2.5 or Wilms tumor 1 (Wt1) and Nkx2.5.

Results

Tbx18 and Wt1, but not Nkx2.5, were expressed in the proepicardium and epicardium. Tbx18 was expressed in cells within the heart from E10.5 to at least E14.5; these Tbx18-expressing cells were Nkx2.5 positive, except for a few cells that were Nkx2.5 negative at E14.5. Wt1 was expressed in cells within the heart from E12.5 to at least E14.5, but these Wt1-expressing cells were Nkx2.5 negative.

Conclusion

The data obtained in this study demonstrate that Tbx18 is expressed in the cardiomyocytes from E10.5 to at least E14.5, and Wt1 is expressed within the heart from E12.5 to at least E14.5, but not in the cardiomyocyte. These findings may provide new insights on the role of the epicardial cells in cardiac regeneration.

【 授权许可】

   
2011 Zeng et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

【 参考文献 】

• [1]Nesbitt T, Lemley A, Davis J, Yost MJ, Goodwin RL, Potts JD: Epicardial development in the rat: a new perspective. Microsc Microanal 2006, 12(5):390-8.
• [2]Ratajska A, Czarnowska E, Ciszek B: Embryonic development of the proepicardium and coronary vessels. Int J Dev Biol 2008, 52:229-236.
• [3]Guadix JA, Carmona R, Muñoz-Chápuli R, Pérez-Pomares JM: In vivo and in vitro analysis of the vasculogenic potential of avian proepicardial and epicardial cells. Dev Dyn 2006, 235:1014-1026.
• [4]Smart N, Risebro CA, Melville AA, Moses K, Schwartz RJ, Chien KR, Rilery PR: Thymosin beta4 induces adult epicardial progenitor mobilization and neovascularization. Nature 2007, 445:177-182.
• [5]Pennisi DJ, Mikawa T: FGFR-1 is required by epicardium-derived cells for myocardial invasion and correct coronary vascular lineage differentiation. Dev Biol 2009, 328:148-159.
• [6]Lepilina A, Coon AN, Kikuchi K, Holdway JE, Roberts RW, Burns CG, Poss KD: A dynamic epicardial injury response supports progenitor cell activity during zebrafish heart regeneration. Cell 2006, 127:607-619.
• [7]Cai CL, Martin JC, Sun Y, Cui L, Wang L, Ouyang K, Yang L, Bu L, Liang X, Zhang X, Stallcup WB, Denton CP, McCulloch A, Chen J, Evans SM: A myocardial lineage derives from Tbx18 epicardial cells. Nature 2008, 454:104-108.
• [8]Zhou B, Ma Q, Rajagopal S, Wu SM, Domian I, Rivera-Feliciano J, Jiang D, Von Gise A, Ikeda S, Chien KR, Pu WT: Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature 2008, 454:109-113.
• [9]Christoffels VM, Grieskamp T, Norden J, Mommersteeg MT, Rudat C, Kispert A: Tbx18 and the fate of epicardial progenitors. Nature 2009, 16:E8-9.
• [10]Zeng B, Lin G, Ren X, Zhang Y, Chen H: Over-expression of HO-1 on mesenchymal stem cells promotes angiogenesis and improves myocardial function in infarcted myocardium. J Biomed Sci 2010, 7:80-88.
• [11]Li Y, Zhang D, Zhang Y, He G, Zhang F: Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation. J Biomed Sci 2010, 17:75-85. BioMed Central Full Text
• [12]Zeng B, Chen H, Zhu C, Ren X, Lin G, Cao F: Effects of combined mesenchymal stem cells and heme oxygenase-1 therapy on cardiac performance. Eur J Cardiothorac Surg 2008, 34:850-6.
• [13]Wu KH, Liu YL, Zhou B, Han ZC: Cellular therapy and myocardial tissue engineering: the role of adult stem and progenitor cells. Eur J Cardiothorac Surg 2006, 30:770-81.
• [14]Siepe M, Akhyari P, Lichtenberg A, Schlensak C, Beyersdorf F: Stem cells used for cardiovascular tissue engineering. Eur J Cardiothorac Surg 2008, 34:242-247.
• [15]Bochmann L, Sarathchandra P, Mori F, Lara-Pezzi E, Lazzaro D, Rosenthal N: Revealing new mouse epicardial cell markers through transcriptomics. PloS One 2010, 28:e11429.
• [16]Lam JT, Moretti A, Laugwitz KL: Multipotent Progenitor Cells in Regenerative Cardiovascular medicine. Pediiatr Cardiol 2009, 30:690-698.
• [17]Ieda M, Tsuchihashi T, Ivey KN, Ross RS, Hong TT, Shaw RM, Srivastava D: Cardiac fibroblasts regulate myocardial proliferation through beta1 integrin signaling. Dev Cell 2009, 16:233-244.
• [18]Harvey RP: NK-2 homeobox genes and heart development. Dev Biol 1996, 178:203-16.
• [19]Zhou B, von Gise A, Ma Q, Rivera-Feliciano J, Pu WT: Nkx2-5- and Isl1-expressing cardiac progenitors contribute to proepicardium. Biochem Biophys Res Commun 2008, 375:450-453.
• [20]Wu SM, Fujiwara Y, Cibulsky SM, Clapham DE, Lien CL, Schultheiss TM, Orkin SH: Developmental origin of a bipotential myocardial and smooth muscle cell precursor in the mammalian heart. Cell 2006, 127:1137-50.
• [21]Snider P, Standley KN, Wang J, Azhar M, Doetschman T, Conway SJ: Origin of cardiac fibroblasts and the role of periostin. Circ Res 2009, 105:934-947.
• [22]Martínez-Estrada OM, Lettice LA, Essafi A, Guadix JA, Slight J, Velecela V, Hall E, Reichmann J, Devenney PS, Hohenstein P, Hosen N, Hill RE, Munoz-Chapuli R, Hastie ND: Wt1 is required for cardiovascular progenitor cell formation through transcriptional control of Snail and E-cadherin. Nat Gent 2010, 42:89-93.