Exploiting Issatchenkia orientalis SD108 for succinic acid production

Microbial Cell Factories

Research

Zengyi Shao¹  Han Xiao¹  Huimin Zhao²  Yu Jiang³  Sudhanshu Dole⁴ 

[1] Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA;Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA;Departments of Chemistry, Biochemistry, and Bioengineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 66 Cummings Park, 61801, Urbana, IL, USA;Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA;Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciencesn, 200032, Shanghai, IL, China;Molecular Biology Department, Myriant Corporation, 66 Cummings Park, 01801, Woburn, MA, England;

关键词: Succinic acid;    Acid tolerance;    Issatchenkia orientalis;    Metabolic engineering;   

DOI  :  10.1186/s12934-014-0121-4

 received in 2014-05-27, accepted in 2014-08-10,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundRecent advances in synthesizing valuable chemicals such as organic acids from low-cost renewable biomass through microbial fermentation have attracted great attention. However, the toxicity of organic acids presents a key challenge to the development of an economically viable fermentation process. Therefore, a platform organism that not only produces organic acids but also tolerates the associated toxicity is highly desirable.ResultsHere we report the discovery, characterization, and engineering of a yeast strain, Issatchenkia orientalis SD108, that is tolerant to low pH and high concentration of organic acids. This strain demonstrated a higher tolerance compared to I. orientalis ATCC 24210 and Classic Distiller’s Turbo yeast. In order to explore SD108 as a potential platform organism for organic acid production, we determined its draft genome sequence and use the sequencing information to guide pathway design. As proof of concept, an engineered four-gene expression cassette related to the reductive TCA cycle was assembled and integrated into the genome of a uracil auxotroph of SD108. The resulting strain was able to produce succinic acid with a titer of 11.63 g/L, yield of 0.12 g/g, and productivity of 0.11 g/L . h in batch cultures using shake flasks.ConclusionsThe high tolerance of I. orientalis SD108 towards multiple important organic acids makes it a highly attractive organism as a platform host for producing this group of compounds as it will reduce production cost, facilitate downstream processing, and serve as a host for construction of production strains with both pH and specific anion tolerance.

【 授权许可】

Unknown   
© Xiao et al.; licensee BioMed Central 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.