MicroRNAs and targets in senescent litchi fruit during ambient storage and post-cold storage shelf life

BMC Plant Biology

Research Article

Yueming Jiang¹  Hong Zhu¹  Hongxia Qu¹  Chun Yi¹  Furong Yao² 

[1] Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, P R of China;Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, P R of China;University of Chinese Academy of Sciences, 100049, Beijing, P R of China;

关键词: Litchi chinensis;    MicroRNAs;    Targets;    Senescence;    Storage;   

DOI  :  10.1186/s12870-015-0509-2

 received in 2014-11-14, accepted in 2015-04-27,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundLitchi has a high commercial value due to its bright color and rich nutrients. However, it deteriorates with the pericarp turning brown within 1–2 days after harvest. The factors that mediate litchi fruit senescence are complicated. MicroRNAs act as negative regulators involved in almost every physiological process. To understand the mechanism of litchi fruit senescence and pericarp browning at the miRNA level, five small RNA libraries and a degradome library prepared from the pericarp of litchi fruit subjected to ambient storage and post-cold storage shelf life were sequenced.ResultsBy aligning the sRNA reads onto the litchi unigene assembly, 296 miRNAs belonging to 49 known miRNA families were first identified from litchi. In addition, 11 litchi-specific miRNAs were identified. Among these, 167 known miRNAs were identified to cleave 197 targets, and three litchi-specific miRNAs were found to have five targets. Through combined analysis of stem-loop quantitative real-time polymerase chain reaction (qRT-PCR) and transcriptome profiling, 14 miRNA-target pairs were found to be actively involved in litchi fruit senescence-related processes, including energy regulation, anthocyanin metabolism, hormone signaling, and pathogen-infection defense.ConclusionsA network of miRNA-targets that regulate litchi fruit senescence has been proposed, revealing the miRNA-mediated regulation in senescent litchi fruit. This will aid in developing new strategies to postpone the senescence of litchi fruit and other horticultural products.

【 授权许可】

Unknown   
© Yao et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.