Investigating the effect of the inhibitory peptide on L.monocytogenes cell invasion: an in silico and in vitro study

Gut Pathogens

Research

Behrooz Sadeghi Kalani¹  Ali Hematian¹  Sara Bahonar²  Ali Shivaee²  Mehdi Goudarzi²  Bahareh Hajikhani³ 

[1] Department of Medical Microbiology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran;Department of Medical Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Department of Medical Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;

关键词: L.monocytogenes monocytogenes;    Internalin A;    E-cadherin;    Molecular docking;    Inhibitory peptide;    Cell line;   

DOI  :  10.1186/s13099-023-00576-7

 received in 2023-07-15, accepted in 2023-09-30,  发布年份 2023

来源: Springer

【 摘 要 】

AimsL.monocytogenes monocytogenes is an omnipresent bacterium that causes a fatal food-borne illness, listeriosis. The connection of this bacterium to E-cadherin through internalin A plays a significant role in the internalization of the bacteria. In this study, this interaction has been investigated for the design of an inhibitory peptide.MethodsThe interaction of the proteins involved in the entry of bacteria was evaluated by molecular docking. According to their interactions, an inhibitory peptide was designed to bind to internalin A by server peptiderive. Its effects on L.monocytogenes invasion on the Caco-2 cell line and biofilm formation were also assessed.FindingsDocking results showed that the peptide has a high affinity for binding to Internalin A. The synthesized peptide at a concentration of 64 µg/ml inhibited 80% of the invasion of L.monocytogenes into the Caco-2 cell line. Furthermore, the studied peptide at the highest concentration had a slight inhibitory effect on biofilm formation.ConclusionThese results reveal that short polypeptides can impede the invasion of target cells by L. monocytogenes in vitro and could be advantageous as restoring agents in vivo.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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