CANDLES, an assay for monitoring GPCR induced cAMP generation in cell cultures

Cell Communication and Signaling

Methodology

Emmi Rotgers¹  Ashutosh Trehan¹  Ilpo Huhtaniemi²  Adolfo Rivero-Müller³  Eleanor T Coffey⁴ 

[1] Department of Physiology, Institute of Biomedicine, University of Turku, Turku, Finland;Department of Physiology, Institute of Biomedicine, University of Turku, Turku, Finland;Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Hammersmith Campus, Imperial College London, London, UK;Department of Physiology, Institute of Biomedicine, University of Turku, Turku, Finland;Faculty of Natural Sciences and Technology, Åbo Akademi University, Turku, Finland;Department of Biochemistry and Molecular Biology, Medical University of Lublin, Lublin, Poland;Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, BioCity, Turku, Finland;

关键词: cAMP;    Bioassay;    GPCR;    Biosensor;    Cell-cell interaction;    Kinetics;    CANDLES;    Gap junctions;    Connexin;    FRET;   

DOI  :  10.1186/s12964-014-0070-x

 received in 2014-06-03, accepted in 2014-10-19,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundG protein-coupled receptors (GPCRs) represent a physiologically and pharmacologically important family of receptors that upon coupling to GαS stimulate cAMP production catalyzed by adenylyl cyclase. Thus, developing assays to monitor cAMP production is crucial to screen for ligands in studies of GPCR signaling. Primary cell cultures represent a more robust model than cell lines to study GPCR signaling since they physiologically resemble the parent tissue. Current cAMP assays have two fundamental limitations: 1) absence of cAMP kinetics as competition-based assays require cell lysis and measure only a single time-point, and 2) high variation with separate samples needed to measure consecutive time points. The utility of real-time cAMP biosensors is also limited in primary cell cultures due to their poor transfection efficiency, variable expression levels and inability to select stable clones. We therefore, decided to develop an assay that can measure cAMP not only at a single time-point but the entire cAMP kinetics after GPCR activation in untransfected primary cells.ResultsCANDLES (C yclic A MP iN direct D etection by L ight E mission from S ensor cells) assay for monitoring cAMP kinetics in cell cultures, particularly in primary cultures was developed. The assay requires co-culturing of primary cells with sensor cells that stably express a luminescent cAMP sensor. Upon GPCR activation in primary cells, cAMP is transferred to sensor cells via gap junction channels, thereby evoking a luminescent read-out. GPCR activation using primary cultures of rat cortical neurons and mouse granulosa cells was measured. Kinetic responses of different agonists to adrenergic receptors were also compared using rat cortical neurons. The assay optimization was done by varying sensor-test cell ratio, using phosphodiesterase inhibitors and testing cell-cell contact requirement.ConclusionsHere we present CANDLES assay based on co-culturing test cells with cAMP-detecting sensor cells. This co-culture setup allows kinetic measurements, eliminates primary cell transfections and reduces variability. A variety of cell types (rat cortical neurons, mouse granulosa cells and established cell lines) and receptors (adrenergic, follicle stimulating hormone and luteinizing hormone/chorionic gonadotropin receptors) were tested for use with CANDLES. The assay is best applied while comparing cAMP generation curves upon different drug treatments to untransfected primary cells.

【 授权许可】

Unknown   
© Trehan et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.