Chronic wounds alter the proteome profile in skin mucus of farmed gilthead seabream

BMC Genomics

Research Article

Monica F. Brinchmann¹  María A. Esteban²  Alberto Cuesta²  Héctor Cordero³ 

[1] Faculty of Biosciences and Aquaculture, Nord University, 8049, Bodø, Norway;Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100, Murcia, Spain;Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100, Murcia, Spain;Department of Microbiology and Immunology, Rosalind Franklin University of Medicine and Science, 60064, North Chicago, IL, USA;Faculty of Biosciences and Aquaculture, Nord University, 8049, Bodø, Norway;

关键词: Proteome;    Wounds;    Skin mucus;    Sparus aurata;    Teleosts;    IgM;   

DOI  :  10.1186/s12864-017-4349-3

 received in 2017-03-10, accepted in 2017-11-24,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundSkin and its mucus are known to be the first barrier of defence against any external stressors. In fish, skin wounds frequently appear as a result of intensive culture and also some diseases have skin ulcers as external clinical signs. However, there is no information about the changes produced by the wounds in the mucosae. In the present paper, we have studied the alterations in the proteome map of skin mucus of gilthead seabream during healing of experimentally produced chronic wounds by 2-DE followed by LC-MS/MS. The corresponding gene expression changes of some identified skin proteins were also investigated through qPCR.ResultsOur study has successfully identified 21 differentially expressed proteins involved in immunity and stress processes as well as other metabolic and structural proteins and revealed, for the first time, that all are downregulated in the skin mucus of wounded seabream specimens. At transcript level, we found that four of nine markers (ighm, gst3, actb and krt1) were downregulated after causing the wounds while the rest of them remained unaltered in the wounded fish. Finally, ELISA analysis revealed that IgM levels were significantly lower in wounded fish compared to the control fish.ConclusionsOur study revealed a decreased-expression at protein and for some transcripts at mRNA levels in wounded fish, which could affect the functionality of these molecules, and therefore, delay the wound healing process and increase the susceptibility to any infection after wounds in the skin of gilthead seabream.

【 授权许可】

CC BY   
© The Author(s). 2017