Novel approach of using a cocktail of designed bacteriophages against gut pathogenic E. colifor bacterial load biocontrol

Annals of Clinical Microbiology and Antimicrobials

Research

Sabah AA Jassim¹  Fatimah Abu Bakar²  Ahmed Sahib Abdulamir³ 

[1] Applied Bio Research Inc, 455 Pelissier St, N9A 6Z9, Ontario, Windsor, Canada;Faculty of Food Science, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Microbiology Department, College of Medicine, Alnahrain University, 14222, Baghdad, Iraq;

关键词: Bacteriophage;    Animal feed;    Biocontrol;    E. coli;    Bioprocessing;    Phage design;    Phage cocktail;   

DOI  :  10.1186/s12941-014-0039-z

 received in 2014-05-09, accepted in 2014-07-19,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThis study was conducted to explore new approaches of animal biocontrol via biological control feed.MethodWhite rats were subjected to 140 highly lytic designed phages specific against E. coli. Phages were fed via drinking water, oral injection, and vegetable capsules. Phage feeding was applied by 24 h feeding with 11d monitoring and 20d phage feeding and monitoring. Group of rats received external pathogenic E. coli and another group did not, namely groups A and B.ResultsPhage feeding for 20d via vegetable capsules yielded the highest reduction of fecal E. coli, 3.02 and 4.62 log, in rats group A and B respectively. Second best, feeding for 20d via drinking water with alkali yielded 2.78 and 4.08 log in rats groups A and B respectively. The peak reduction in E. coli output was 5–10 d after phage feeding. Phage control declined after 10th day of feeding.ConclusionsThe use of cocktail of designed phages succeeded in suppressing flora or external E. coli. The phage feed biocontrol is efficient in controlling E. coli at the pre-harvest period, precisely at the 6th-8th day of phage feeding when the lowest E. coli output found.

【 授权许可】

CC BY   
© Abdulamir et al.; licensee BioMed Central Ltd. 2014