Carotenoid accumulation affects redox status, starch metabolism, and flavonoid/anthocyanin accumulation in citrus

BMC Plant Biology

Research Article

Xintian Dong¹  Yan Han¹  Haijiang Chen¹  Fei Zhao²  Xiuxin Deng²  Qiaoli Ma²  Yuduan Ding²  Qiang Xu²  Juan Xu²  Hongbo Cao³  Jiangbo Wang⁴  Jiancheng Zhang⁵ 

[1] College of Horticulture, Agricultural University of Hebei, 071001, Baoding, Hebei, China;Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, 430070, Wuhan, Hubei, China;Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, 430070, Wuhan, Hubei, China;College of Horticulture, Agricultural University of Hebei, 071001, Baoding, Hebei, China;Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, 430070, Wuhan, Hubei, China;Present address: College of Plant Science, Tarim University, 843300, Alar, China;Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, 430070, Wuhan, Hubei, China;Present address: Shanxi Agricultural University, 030801, Taigu, Shanxi, China;

关键词: Carotenogenesis;    Citrus;    Redox status;    Starch;    Chromoplast;    Anthocyanin;   

DOI  :  10.1186/s12870-015-0426-4

 received in 2014-09-18, accepted in 2015-01-15,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundCarotenoids are indispensable plant secondary metabolites that are involved in photosynthesis, antioxidation, and phytohormone biosynthesis. Carotenoids are likely involved in other biological functions that have yet to be discovered. In this study, we integrated genomic, biochemical, and cellular studies to gain deep insight into carotenoid-related biological processes in citrus calli overexpressing CrtB (phytoene synthase from Pantoea agglomerans). Fortunella hindsii Swingle (a citrus relative) and Malus hupehensis (a wild apple) calli were also utilized as supporting systems to investigate the effect of altered carotenoid accumulation on carotenoid-related biological processes.ResultsTranscriptomic analysis provided deep insight into the carotenoid-related biological processes of redox status, starch metabolism, and flavonoid/anthocyanin accumulation. By applying biochemical and cytological analyses, we determined that the altered redox status was associated with variations in O2- and H2O2 levels. We also ascertained a decline in starch accumulation in carotenoid-rich calli. Furthermore, via an extensive cellular investigation of the newly constructed CrtB overexpressing Fortunella hindsii Swingle, we demonstrated that starch level reducation occurred in parallel with significant carotenoid accumulation. Moreover, studying anthocyanin-rich Malus hupehensis calli showed a negative effect of carotenoids on anthocyanin accumulation.ConclusionsIn citrus, altered carotenoid accumulation resulted in dramatic effects on metabolic processes involved in redox modification, starch degradation, and flavonoid/anthocyanin biosynthesis. These findings provided new perspectives to understand the biological importance of carotenogenesis and of the developmental processes associated with the nutritional and sensory qualities of agricultural products that accumulate carotenoids.

【 授权许可】

Unknown   
© Cao et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.