CLASP2 is involved in the EMT and early progression after transurethral resection of the bladder tumor

BMC Cancer

Research Article

Jun Wang¹  Wentao Liu²  Lin Qi³  Yuan Li³  Longfei Liu³  Zhenyu Ou³  Minfeng Chen³  Xiongbing Zu³  Sulai Liu³  Bisong Zhu³ 

[1] Department of Urology, The first affiliated Hospital, Zhengzhou University, No.1 Jianshe Dong Road, 450000, Zhengzhou, People’s Republic of China;Department of Urology, The second Xiangya Hospital, Central South University, Renmin Road, 410000, Changsha, People’s Republic of China;Department of Urology, Xiangya Hospital, Central South University, No. 87 Xiangya Road, 410008, Changsha, Hunan Province, People’s Republic of China;

关键词: CLASP2 protein;    Cell proliferation;    Disease progression;    Epithelial-mesenchymal transition;    Urinary bladder neoplasms;   

DOI  :  10.1186/s12885-017-3101-3

 received in 2015-12-21, accepted in 2017-02-01,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundCytoplasmic linker-associated protein 2 (CLASP2) belongs to a family of microtubule plus-end tracking proteins that localizes to the distal ends of microtubules and regulate microtubule dynamics. We speculated that it might be involved in the epithelial-mesenchymal transition (EMT) and progression of bladder cancer (BC).MethodsWestern blotting and RT-PCR were used to detect the changes at protein and mRNA levels in BC cell lines. Cell proliferation, clonogenic formation, wound healing and chamber invasion assay were used to investigate the abilities of cellular proliferation, migration and invasion. The data of BC patients treated with transurethral resection of the bladder tumor (TURBT) was collected and analyzed. The levels of mRNA of CLASP2 and EMT-related markers in tumor and urine samples were tested by RT-PCR.ResultsExpressions of CLASP2 varied in four BC cell lines. Manipulation of CLASP2 expression changed EMT-related markers. CLASP2 could promote proliferation, migration and invasion in BC cell lines. The combination (CLASP2 + E-cadherin mRNA in urine) could better discriminate the patients with or without 2-years progression compared with tumor grade after TURBT.ConclusionCLASP2 is involved in the EMT and progression of bladder urothelial cancer. Simultaneous urine-based detection of CLASP2 and E-cadherin mRNA can efficiently discriminate patients with or without 2-years progression after TURBT.

【 授权许可】

CC BY   
© The Author(s). 2017