Journal of Inflammation | |
Hydrogen-rich saline attenuates eosinophil activation in a guinea pig model of allergic rhinitis via reducing oxidative stress | |
Research | |
Na Che1  Lin Jing1  Rongming Ge1  Shaoqing Yu1  Chuanliang Zhao1  | |
[1] Department of Otolaryngology, Tongji Hospital, Tongji University, 389 Xincun road, Putuo District, 200065, Shanghai, China; | |
关键词: Allergic rhinitis; Reactive oxygen species; Hydrogen-rich saline; Eosinophil; Eosinophil cationic protein; Eotaxin; Guinea pig; | |
DOI : 10.1186/s12950-016-0148-x | |
received in 2016-11-03, accepted in 2016-12-09, 发布年份 2017 | |
来源: Springer | |
【 摘 要 】
BackgroundIt is well considered that reactive oxygen species (ROS) plays a prominent causative role in the development of allergic rhinitis (AR), and eosinophils cells as important allergic inflammatory cells contribute to elevating oxidative stress. Hydrogen, emerging as a novel antioxidant, has been proven effective in selectively reducing ROS in animals models of oxidative damage. We herein aim to verify protective effects of hydrogen on eosinophils cells in guinea pigs models of AR.MethodsThirty two guinea pigs were random divided into four groups, and AR model was established through ovalbumin sensitization. The guinea pigs were injected with hydrogen-rich saline (Normal-HRS and AR-HRS group) or normal saline (control and AR group). The frequencies of sneezing and scratching were recorded. The IgE level, blood eosinophil count and eosinophil cationic protein (ECP) level in serum were measured. The serum malondialdehyde (MDA) and superoxide dismutase (SOD) assays were also measured to evaluate oxidative stress. The expression levels of eotaxin mRNA and protein in the nasal mucosa were also determined by real-time RT-PCR, Western blot and immunofluorescence.ResultsHRS reduced the ROS and MDA levels and increased SOD level in guinea pigs of AR-HRS group accompanied with decreased frequency of sneezing and scratches. Meanwhile, there was a decline of the number of eosinophils cells in blood and of thelevel of ECP in serum in the AR-HRS group. HRS also significantly decreased the expression of eotaxin in nasal mucosa.ConclusionHRS may play a protective role in attenuating allergic inflammation, and suppressing the increase and activation of eosinophils in AR possibly through antioxidation effect of hydrogen.
【 授权许可】
CC BY
© The Author(s). 2017
【 预 览 】
Files | Size | Format | View |
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RO202311108110663ZK.pdf | 1520KB | download |
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