Effects of nutritional conditions on growth and biochemical composition of Tetraselmis sp.

Lipids in Health and Disease

Research

Imen Fendri¹  Mohamed Barkallah²  Ramzi Miladi²  Slim Abdelkafi²  Faiez Hentati²  Bilel Hadrich²  Mouna Dammak²  Ridha Hachicha²  Céline Laroche³  Philippe Michaud³ 

[1] Laboratory of Plant Biotechnology, Faculty of Sciences of Sfax, University of Sfax, Sfax, Tunisia;Unité de Biotechnologie des Algues, Biological Engineering Department, National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia;Université Clermont Auvergne, Université Blaise Pascal, Institut Pascal, BP 10448, F-63000, Clermont-Ferrand, France;CNRS, UMR 6602, IP, F-63178, Aubière, France;

关键词: Microalgae;    Tetraselmis;    Starch;    Biomass;    Bioactive compounds;    Box-Behnken Design;   

DOI  :  10.1186/s12944-016-0378-1

 received in 2016-11-07, accepted in 2016-11-24,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThis study aimed to maximize biomass concentration, biomass productivity and biochemical composition of the marine microalga Tetraselmis sp.MethodsIn the current study, Box-Behnken Design was used to model the effect of NaNO3, NaH2PO4, metals and vitamins in the F/2 medium on the growth, total chlorophylls, carotenoids and starch contents. The total chlorophylls content was quantified by spectrophotometry. The FT-IR spectroscopy was used to estimate the biochemical compositions of Tetraselmis sp. grown under both optimized medium culture for starch production and standard culture medium.ResultsFinalized NaNO3 (1.76 mM), NaH2PO4 (0.018 mM), metals (1500 μL.L−1) and vitamins (312.5 μL.L−1) concentrations, generated an increase in biomass concentration up to 5.72 g.L−1 which contributed to an increase about 2.4-fold than that of the standard conditions of biomass productivity (408.57 mg.L−1.day−1). The maximum value of carotenoids content (0.3 mg.g DW−1) was achieved at the highest level of all factors. The total chlorophylls content reached also its maximum (5.18 mg.g DW−1) at high nitrate (1.76 mM), phosphate (0.054 mM), metals and vitamins concentrations, while the maximum starch content (42% DW) was achieved with low nitrate and phosphate concentrations (0.58 mM and 0.027 mM) and with metals and vitamins limitations. Thus, the nitrogen, phosphorus, metals and vitamins limitations led to divert the metabolism for the starch biosynthesis.ConclusionsThe high biomass concentration productivity and starch production make Tetraselmis sp. strain a good candidate for biotechnological applications.

【 授权许可】

CC BY   
© The Author(s). 2016