Aluminium-phthalocyanine chloride nanoemulsions for anticancer photodynamic therapy: Development and in vitro activity against monolayers and spheroids of human mammary adenocarcinoma MCF-7 cells

Journal of Nanobiotechnology

Research

Mônica Pereira Garcia¹  Mosar Corrêa Rodrigues¹  Karen Rapp Py-Daniel¹  Ricardo Bentes Azevedo¹  João Paulo Figueiró Longo¹  Sebastião William da Silva²  Paulo Eduardo Narciso de Souza²  Aline Bessa Veloso²  Luis Alexandre Muehlmann³ 

[1] Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Federal District, Brazil;Department of Physics, University of Brasilia, Federal District, Brazil;Faculty of Ceilandia, University of Brasilia, Federal District, Brazil;

关键词: Spontaneous emulsification;    Cancer;    Drug delivery systems;    Third-generation photosensitizers;    Human breast adenocarcinoma MCF-7 cells;    Spheroids;   

DOI  :  10.1186/s12951-015-0095-3

 received in 2015-02-26, accepted in 2015-05-01,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundPhotodynamic therapy (PDT) combines light, molecular oxygen and a photosensitizer to induce oxidative stress in target cells. Certain hydrophobic photosensitizers, such as aluminium-phthalocyanine chloride (AlPc), have significant potential for antitumor PDT applications. However, hydrophobic molecules often require drug-delivery systems, such as nanostructures, to improve their pharmacokinetic properties and to prevent aggregation, which has a quenching effect on the photoemission properties in aqueous media. As a result, this work aims to develop and test the efficacy of an AlPc in the form of a nanoemulsion to enable its use in anticancer PDT.ResultsThe nanoemulsion was developed using castor oil and Cremophor ELP®, and a monodisperse population of nanodroplets with a hydrodynamic diameter of approximately 25 nm was obtained. While free AlPc failed to show significant activity against human breast adenocarcinoma MCF-7 cells in an in vitro PDT assay, the AlPc in the nanoemulsion showed intense photodynamic activity. Photoactivated AlPc exhibited a 50 % cytotoxicity concentration (CC50) of 6.0 nM when applied to MCF-7 cell monolayers and exerted a powerful cytotoxic effect on MCF-7 cell spheroids.ConclusionThrough the use of spontaneous emulsification, a stable AlPc nanoemulsion was developed that exhibits strong in vitro photodynamic activity on cancer cells.

【 授权许可】

Unknown   
© Muehlmann et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.