The long noncoding RNA SPRY4-IT1 increases the proliferation of human breast cancer cells by upregulating ZNF703 expression

Molecular Cancer

Research

Juan Li¹  Li Wang¹  Keming Wang¹  Yingrui Fan¹  Yun Tian¹  Yifan Lian¹  Jie Ding¹  Yongguo Shi²  Yongqian Shu³  Yangchen Liu⁴ 

[1] Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China;Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China;Taixing People’s Hospital, Taixing, Jiangsu, PR China;Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China;Taixing People’s Hospital, Taixing, Jiangsu, PR China;

关键词: SPRY4-IT1;    ZNF703;    Proliferation;    Breast cancer;   

DOI  :  10.1186/s12943-015-0318-0

 received in 2014-11-02, accepted in 2015-02-09,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundLong noncoding RNAs (lncRNAs) have emerged recently as a new class of genes that regulate cellular processes, such as cell growth and apoptosis. The SPRY4 intronic transcript 1 (SPRY4-IT1) is a 708-bp lncRNA on chromosome 5 with a potential functional role in tumorigenesis. The clinical significance of SPRY4-IT1 and the effect of SPRY4-IT1 on cancer progression are unclear.MethodsQuantitative reverse transcriptase PCR (qRT-PCR) was performed to investigate the expression of SPRY4-IT1 in 48 breast cancer tissues and four breast cancer cell lines. Gain and loss of function approaches were used to investigate the biological role of SPRY4-IT1 in vitro. Microarray bioinformatics analysis was performed to identify the putative targets of SPRY4-IT1, which were further verified by rescue experiments, and by western blotting and qRT-PCR.ResultsSPRY4-IT1 expression was significantly upregulated in 48 breast cancer tumor tissues comparedwith normal tissues. Additionally, increased SPRY4-IT1 expression was found to be associated with a larger tumor size and an advanced pathological stage in breast cancer patients. The knockdown of SPRY4-IT1 significantly suppressed proliferation and caused apoptosis of breast cancer cells in vitro. Furthermore, we discovered that ZNF703 was a target of SPRY4-IT1 and was downregulated by SPRY4-IT1 knockdown. Moreover, we provide the first demonstration that ZNF703 plays an oncogenic role in ER (−) breast carcinoma cells.ConclusionsSPRY4-IT1 is a novel prognostic biomarker and a potential therapeutic candidate for breast cancer.

【 授权许可】

Unknown   
© Shi et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.