Daucus carota pentane-based fractions arrest the cell cycle and increase apoptosis in MDA-MB-231 breast cancer cells

BMC Complementary and Alternative Medicine

Mirvat El-Sibai³  Costantine F Daher³  Robin I Taleb³  Anthony Mansour²  Kikki Bodman-Smith¹  Mohammad Mroueh⁴  Wassim N Shebaby¹ 

[1] Department of Microbial and Cellular Sciences, Faculty of Health and Medical Sciences, University of Surrey, Surrey, UK;School of Medicine, Lebanese American University, Byblos, Lebanon;Department of Natural Sciences, School of Arts and Sciences, Lebanese American University, P.O. Box 36, Byblos, Lebanon;School of Pharmacy, Lebanese American University, P.O. Box 36, Byblos, Lebanon

关键词: MAPK;    PI3K;    Apoptosis;    Proliferation;    Breast cancer;    Daucus carota;   

Others  :  1086165
DOI  :  10.1186/1472-6882-14-387

 received in 2014-02-14, accepted in 2014-10-01,  发布年份 2014

【 摘 要 】

Background

Daucus carota L.ssp.carota (wild carrot), an herb used in folk medicine worldwide, was recently demonstrated to exhibit anticancer activity. In this study we examined the anticancer effect of Daucus carota oil extract (DCOE) fractions on the human breast adenocarcinoma cell lines MDA-MB-231 and MCF-7 and clarified the mechanism of action.

Methods and results

Using the WST assay, the pentane fraction (F1) and 1:1 pentane:diethyl ether fraction (F2) were shown to possess the highest cytotoxicity against both cell lines. Flow cytometric analysis revealed that both fractions induced the accumulation of cells in the sub-G1 phase, increase in apoptotic cell death and chromatin condensation. The increase in apoptosis in response to treatment was also apparent in the increase in BAX and the decrease in Bcl-2 levels as well as the proteolytic cleavage of both caspase-3 and PARP as revealed by Western blot. Furthermore, treatment of MDA-MB-231 cells with either fraction significantly reduced the level of phosphorylated Erk but did not show any effect on phosphorylated Akt. The combined treatment with a potent PI3K inhibitor (wortmannin) and F1 or F2 fraction had a synergistic inhibitory effect on cell survival which shows that these two drugs work on different pathways.

Conclusions

These results suggest that the pentane-based fractions of DCOE possess potential anti-cancer activity that ismainly mediated through the Erk pathway.

【 授权许可】

   
2014 Shebaby et al.; licensee BioMed Central Ltd.

【 图 表 】

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