Novel metal allergy patch test using metal nanoballs

Journal of Nanobiotechnology

Short Communication

Toshio Hongo¹  Takahiro Wada¹  Motohiro Uo¹  Tomoko Sugiyama²  Mikio Kusama²  Yoshiyuki Mori²  Daisuke Omagari³  Kazuo Komiyama³  Heishichiro Takahashi⁴  Hitoshi Sasaki⁵ 

[1] Advanced Biomaterials Department, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, 113-8549, Tokyo, Japan;Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, 3311-1 Yakushiji, 329-0498, Shimotsuke, Tochigi, Japan;Department of Pathology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 131-8310, Tokyo, Japan;Graduate School of Engineering, Hokkaido University, North 13, West 8, 060-8628, Sapporo, Hokkaido, Japan;Nakayamagumi Co. Ltd., North 19, East 1, Higashi-ku, 065-8610, Sapporo, Hokkaido, Japan;

关键词: Nanoparticle;    Nickel;    Metal allergy;    Patch test;    Elemental distribution;   

DOI  :  10.1186/s12951-014-0051-7

 received in 2014-09-15, accepted in 2014-11-06,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundPatch tests are often used in the clinical diagnosis of metal allergies. In currently available patch tests, high concentrations of metal salt solutions are used. However, diagnosis accuracy can be influenced not only by acute skin reactions to high concentrations of metal salt, but also by skin reactions to other components present in the patch or to pH changes. In this study, we developed Ni nanoparticles (termed “nanoballs”) for use in patch-test solutions.FindingsHighly soluble, spherical Ni nanoballs were prepared using plasma electrolysis. The Ni released from the nanoballs permeated through a dialysis membrane, and the nanoball-containing solution’s pH was maintained constant. Ni ions were released slowly at low concentrations in a time-dependent manner, which contrasted the rapid release observed in the case of a commercial patch test. Consequently, in the new test system, reactions caused by high concentrations of metal salts were avoided.ConclusionsBy exploiting the high specific surface area of Ni nanoballs, we obtained an effective dissolution of Ni ions that triggered Ni allergy in the absence of direct contact between the nanoballs and mouse skin. This novel patch system can be applied to other metals and alloys for diagnosing various types of metal-induced contact dermatitis.

【 授权许可】

Unknown   
© Sugiyama et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.