Novel metal allergy patch test using metal nanoballs

Journal of Nanobiotechnology

Yoshiyuki Mori¹  Mikio Kusama¹  Heishichiro Takahashi⁴  Hitoshi Sasaki²  Kazuo Komiyama⁵  Daisuke Omagari⁵  Toshio Hongo³  Takahiro Wada³  Motohiro Uo³  Tomoko Sugiyama¹ 

[1]Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke 329-0498, Tochigi, Japan

[2]Nakayamagumi Co. Ltd., North 19, East 1, Higashi-ku, Sapporo 065-8610, Hokkaido, Japan

[3]Advanced Biomaterials Department, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku 113-8549, Tokyo, Japan

[4]Graduate School of Engineering, Hokkaido University, North 13, West 8, Sapporo 060-8628, Hokkaido, Japan

[5]Department of Pathology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku 131-8310, Tokyo, Japan

关键词: Elemental distribution;    Patch test;    Metal allergy;    Nickel;    Nanoparticle;   

Others  :  1139308
DOI  :  10.1186/s12951-014-0051-7

 received in 2014-09-15, accepted in 2014-11-06,  发布年份 2014

【 摘 要 】

Background

Patch tests are often used in the clinical diagnosis of metal allergies. In currently available patch tests, high concentrations of metal salt solutions are used. However, diagnosis accuracy can be influenced not only by acute skin reactions to high concentrations of metal salt, but also by skin reactions to other components present in the patch or to pH changes. In this study, we developed Ni nanoparticles (termed “nanoballs”) for use in patch-test solutions.

Findings

Highly soluble, spherical Ni nanoballs were prepared using plasma electrolysis. The Ni released from the nanoballs permeated through a dialysis membrane, and the nanoball-containing solution’s pH was maintained constant. Ni ions were released slowly at low concentrations in a time-dependent manner, which contrasted the rapid release observed in the case of a commercial patch test. Consequently, in the new test system, reactions caused by high concentrations of metal salts were avoided.

Conclusions

By exploiting the high specific surface area of Ni nanoballs, we obtained an effective dissolution of Ni ions that triggered Ni allergy in the absence of direct contact between the nanoballs and mouse skin. This novel patch system can be applied to other metals and alloys for diagnosing various types of metal-induced contact dermatitis.

【 授权许可】

   
2014 Sugiyama et al.; licensee BioMed Central Ltd.

【 图 表 】

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