Identification of differentially expressed genes associated with semigamy in Pima cotton (Gossypium barbadenseL.) through comparative microarray analysis

BMC Plant Biology

Research Article

J McD Stewart¹  Jinfa Zhang²  Laura Rodriguez-Uribe²  Jessica Curtiss² 

[1] Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 72701, Fayetteville, AR, USA;Department of Plant and Environmental Sciences, New Mexico State University, 88003, Las Cruces, NM, USA;

关键词: Gossypol;    Ovule Tissue;    Differentially Express;    Rubisco Activase;    Phosphoethanolamine;   

DOI  :  10.1186/1471-2229-11-49

 received in 2010-09-12, accepted in 2011-03-16,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundSemigamy in cotton is a type of facultative apomixis controlled by an incompletely dominant autosomal gene (Se). During semigamy, the sperm and egg cells undergo cellular fusion, but the sperm and egg nucleus fail to fuse in the embryo sac, giving rise to diploid, haploid, or chimeric embryos composed of sectors of paternal and maternal origin. In this study we sought to identify differentially expressed genes related to the semigamy genotype by implementing a comparative microarray analysis of anthers and ovules between a non-semigametic Pima S-1 cotton and its doubled haploid natural isogenic mutant semigametic 57-4. Selected differentially expressed genes identified by the microarray results were then confirmed using quantitative reverse transcription PCR (qRT-PCR).ResultsThe comparative analysis between isogenic 57-4 and Pima S-1 identified 284 genes in anthers and 1,864 genes in ovules as being differentially expressed in the semigametic genotype 57-4. Based on gene functions, 127 differentially expressed genes were common to both semigametic anthers and ovules, with 115 being consistently differentially expressed in both tissues. Nine of those genes were selected for qRT-PCR analysis, seven of which were confirmed. Furthermore, several well characterized metabolic pathways including glycolysis/gluconeogenesis, carbon fixation in photosynthetic organisms, sesquiterpenoid biosynthesis, and the biosynthesis of and response to plant hormones were shown to be affected by differentially expressed genes in the semigametic tissues.ConclusionAs the first report using microarray analysis, several important metabolic pathways affected by differentially expressed genes in the semigametic cotton genotype have been identified and described in detail. While these genes are unlikely to be the semigamy gene itself, the effects associated with expression changes in those genes do mimic phenotypic traits observed in semigametic plants. A more in-depth analysis of semigamy is necessary to understand its expression and regulation at the genetic and molecular level.

【 授权许可】

Unknown   
© Curtiss et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.