A plant natriuretic peptide-like molecule of the pathogen Xanthomonas axonopodis pv. citricauses rapid changes in the proteome of its citrus host

BMC Plant Biology

Research Article

Ludivine Thomas¹  Bongani Ndimba¹  Chris Gehring²  Lucas D Daurelio³  Elena G Orellano³  Jorgelina Ottado³  Tamara Zimaro³  Natalia Gottig³  Betiana S Garavaglia⁴ 

[1] Department of Biotechnology, University of the Western Cape, 7535, Bellville, South Africa;Department of Biotechnology, University of the Western Cape, 7535, Bellville, South Africa;CBRC, 4700 King Abdullah University of Science and Technology, 23955-6900, Thuwal, Kingdom of Saudi Arabia;Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, (S2002LRK), Rosario, Argentina;Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, (S2002LRK), Rosario, Argentina;Consejo de Investigaciones de la Universidad Nacional de Rosario, Rosario, Argentina;

关键词: Salicylic Acid;    Photosynthetic Efficiency;    Chlorophyll Fluorescence Parameter;    Rubisco Activase;    Biotrophic Pathogen;   

DOI  :  10.1186/1471-2229-10-51

 received in 2009-08-06, accepted in 2010-03-21,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPlant natriuretic peptides (PNPs) belong to a novel class of peptidic signaling molecules that share some structural similarity to the N-terminal domain of expansins and affect physiological processes such as water and ion homeostasis at nano-molar concentrations. The citrus pathogen Xanthomonas axonopodis pv. citri possesses a PNP-like peptide (XacPNP) uniquely present in this bacteria. Previously we observed that the expression of XacPNP is induced upon infection and that lesions produced in leaves infected with a XacPNP deletion mutant were more necrotic and lead to earlier bacterial cell death, suggesting that the plant-like bacterial PNP enables the plant pathogen to modify host responses in order to create conditions favorable to its own survival.ResultsHere we measured chlorophyll fluorescence parameters and water potential of citrus leaves infiltrated with recombinant purified XacPNP and demonstrate that the peptide improves the physiological conditions of the tissue. Importantly, the proteomic analysis revealed that these responses are mirrored by rapid changes in the host proteome that include the up-regulation of Rubisco activase, ATP synthase CF1 α subunit, maturase K, and α- and β-tubulin.ConclusionsWe demonstrate that XacPNP induces changes in host photosynthesis at the level of protein expression and in photosynthetic efficiency in particular. Our findings suggest that the biotrophic pathogen can use the plant-like hormone to modulate the host cellular environment and in particular host metabolism and that such modulations weaken host defence.

【 授权许可】

Unknown   
© Garavaglia et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.