A stable JAZ protein from peach mediates the transition from outcrossing to self-pollination

BMC Biology

Research Article

Prakash Kumar¹  Pratibha Ravindran¹  Jaideep Mathur²  Gopinadhan Paliyath³  Subramanian Jayasankar⁴  Islam El-Sharkawy⁵  Sherif Sherif⁶ 

[1] Department of Biological Sciences, National University of Singapore, Science Drive 4, 117543, Singapore, Singapore;Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, N1G2W1, Guelph, Ontario, Canada;Department of Plant Agriculture, University of Guelph, 50 Stone Road East, N1G2W1, Guelph, Ontario, Canada;Vineland Research Station, Department of Plant Agriculture, University of Guelph, P.O. Box 7000, 4890 Victoria Av. N, L0R 2E0, Vineland Station, ON, Canada;Vineland Research Station, Department of Plant Agriculture, University of Guelph, P.O. Box 7000, 4890 Victoria Av. N, L0R 2E0, Vineland Station, ON, Canada;Department of Horticulture, Faculty of Agriculture, Damanhour University, PO Box 22516, Al-Gomhuria St, Damanhour, Al-Behira, Egypt;Vineland Research Station, Department of Plant Agriculture, University of Guelph, P.O. Box 7000, 4890 Victoria Av. N, L0R 2E0, Vineland Station, ON, Canada;Department of Plant Agriculture, University of Guelph, 50 Stone Road East, N1G2W1, Guelph, Ontario, Canada;Department of Horticulture, Faculty of Agriculture, Damanhour University, PO Box 22516, Al-Gomhuria St, Damanhour, Al-Behira, Egypt;

关键词: JAZ proteins;    Jasmonic acid;    Autogamy;    Cleistogamy;    Peach;    Floral display;   

DOI  :  10.1186/s12915-015-0124-6

 received in 2014-11-04, accepted in 2015-01-27,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundVariations in floral display represent one of the core features associated with the transition from allogamy to autogamy in angiosperms. The promotion of autogamy under stress conditions suggests the potential involvement of a signaling pathway with a dual role in both flower development and stress response. The jasmonic acid (JA) pathway is a plausible candidate to play such a role because of its involvement in many plant responses to environmental and developmental cues. In the present study, we used peach (Prunus persica L.) varieties with showy and non-showy flowers to investigate the role of JA (and JA signaling suppressors) in floral display.ResultsOur results show that PpJAZ1, a component of the JA signaling pathway in peach, regulates petal expansion during anthesis and promotes self-pollination. PpJAZ1 transcript levels were higher in petals of the non-showy flowers than those of showy flowers at anthesis. Moreover, the ectopic expression of PpJAZ1 in tobacco (Nicotiana tabacum L.) converted the showy, chasmogamous tobacco flowers into non-showy, cleistogamous flowers. Stability of PpJAZ1 was confirmed in vivo using PpJAZ1-GFP chimeric protein. PpJAZ1 inhibited JA-dependent processes in roots and leaves of transgenic plants, including induction of JA-response genes to mechanical wounding. However, the inhibitory effect of PpJAZ1 on JA-dependent fertility functions was weaker, indicating that PpJAZ1 regulates the spatial localization of JA signaling in different plant organs. Indeed, JA-related genes showed differential expression patterns in leaves and flowers of transgenic plants.ConclusionsOur results reveal that under stress conditions – for example, herbivore attacks -- stable JAZ proteins such as PpJAZ1 may alter JA signaling in different plant organs, resulting in autogamy as a reproductive assurance mechanism. This represents an additional mechanism by which plant hormone signaling can modulate a vital developmental process in response to stress.

【 授权许可】

Unknown   
© Sherif et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.