Genome-wide analysis of gene expression reveals gene regulatory networks that regulate chasmogamous and cleistogamous flowering in Pseudostellaria heterophylla (Caryophyllaceae)

BMC Genomics

Research Article

Yin-Ling Luo¹  Bao-Hua Song²  Jin-Yong Hu³  Peng-Fei Wang⁴  Yan Luo⁴  Lu Li⁵ 

[1] College of biology and chemistry, Puer University, 665000, Puer, Yunnan, China;Department of Biological Sciences, University of North Carolina at Charlotte, 28223, Charlotte, NC, USA;Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Lanheiroad 132, 650201, Kunming, Yunnan, China;Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, 666303, Mengla, Yunnan, China;Yunnan Academy of Biodiversity, Southwest Forestry University, Bailongsi 300, 650224, Kunming, Yunnan, China;

关键词: RNA-seq;    Transcriptome;    Cleistogamy;    Cleistogamous flowers;    Chasmogamous flowers;    Environmental response;   

DOI  :  10.1186/s12864-016-2732-0

 received in 2015-04-26, accepted in 2016-05-12,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundPseudostellaria heterophylla produces both closed (cleistogamous, CL) and open (chasmogamous, CH) flowers on the same individual but in different seasons. The production of CH and CL flowers might be in response to environmental changes. To better understand the molecular mechanisms of CH and CL flowering, we compared the transcriptome of the two types of flowers to examine differential gene expression patterns, and to identify gene regulatory networks that control CH and CL flowering.ResultsUsing RNA sequencing, we identified homologues of 428 Arabidopsis genes involved in regulating flowering processes and estimated the differential gene expression patterns between CH and CL flowers. Some of these genes involved in gene regulatory networks of flowering processes showed significantly differential expression patterns between CH and CL flowers. In addition, we identified another 396 differentially expressed transcripts between CH and CL flowers. Some are involved in environmental stress responses and flavonoid biosynthesis.ConclusionsWe propose how the differential expression of key members of three gene regulatory modules may explain CH and CL flowering. Future research is needed to investigate how the environment impinges on these flowering pathways to regulate CH and CL flowering in P. heterophylla.

【 授权许可】

CC BY   
© Luo et al. 2016