RNA-Seq transcriptome data of human cells infected with influenza A/Puerto Rico/8/1934 (H1N1) virus

Data in Brief

Mariia Sergeeva¹  Rinat Amirkhanov²  Evgenii Zhuravlev³  Sergey Malanin³  Tatiana Grigoryeva³  Andrey Komissarov³  Grigory Stepanov⁴  Dmitriy Semenov⁴ 

[1] Corresponding authors.;Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian Federation, St. Petersburg, Russia;Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia;Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia;

关键词: RNA-seq;    Influenza A virus;    H1N1;    Human cells;    Transcriptome;   

DOI  :  

来源: DOAJ

【 摘 要 】

Human influenza remains a serious public health problem. This data article reports the transcriptome analysis data of human cell lines infected with influenza A/Puerto Rico/8/1934 (H1N1) virus. Mock-infected cells were included as controls. Human embryonic fibroblasts (MRC-5) and immortalized cell lines (A549, HEK293FT, WI-38 VA-13) were selected for RNA sequencing using Illumina NextSeq500 platform. Raw data were applied to the bioinformatic pipeline, which includes quality control with FastQC and MultiQC, adapter and quality trimming with Cutadapt, filtering to the genome of influenza A with STAR, transcript quantification with Salmon tool (GRCh38_RefSeq_Transcripts). Differential expressed genes were identified using R package DESeq2 with FDR-adjusted p-value < 0.001 and absolute value of log2(FC) > 1. Lists of differentially expressed genes is provided. The raw and processed RNA-seq data presented in this article were deposited to the European Nucleotide Archive via the ArrayExpress partner repository with the dataset accession number E-MTAB-9511 .

【 授权许可】

Unknown