Bioactivity of Samsum ant (Pachycondyla sennaarensis) venom against lipopolysaccharides through antioxidant and upregulation of Akt1 signaling in rats

Lipids in Health and Disease

Research

Ahmed M Isa¹  Saad Gadoa²  Mohamed Al-Khalifa²  Hossam Ebaid³ 

[1] Department of OB GYNE, College of Medicine, King Saud University, P.O.Box 7805, 11472, Riyadh, Saudi Arabia;Department of Zoology, College of Science, King Saud University, P.O.Box 2455, 11451, Riyadh, Saudi Arabia;Department of Zoology, College of Science, King Saud University, P.O.Box 2455, 11451, Riyadh, Saudi Arabia;Department of Zoology, Faculty of ScienceEl-Minia University, Minya, Egypt;

关键词: AKT1;    Samsum ant venom;    Oxidative stress;    Anti-inflammatory;    Lipopolysaccharides;   

DOI  :  10.1186/1476-511X-11-93

 received in 2012-04-24, accepted in 2012-07-03,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThis study aimed at investigating the oxidative stress ameliorating effect, lipids profile restoration, and the anti-inflammatory effect of Samsum Ant Venom (SAV) in induced endotoxemic male rats, injected with bacterial lipopolysaccharides (LPS).ResultsResults revealed that LPS significantly increased the oxidative stress indications in LPS-injected rats. A significant increase of both malondialdehyde (MDA), and advanced oxidative protein products (AOPP), as well as a significant suppression of glutathione were all detected. Treatment with 100 μg/kg dose of SAV significantly restored the oxidative stress normal indications and increased the total glutathione levels. Treatment of the LPS-rats with 100 μg/kg dose of SAV showed a clear anti-inflammatory function; as the histological architecture of the hepatic tissue was partially recovered, along with a valuable decrease in the leukocytes infiltrated the hepatic tissues. Treatment of some rat groups with 600 μg/kg dose of SAV after LPS injection induced a severe endotoxemia that resulted in very high mortality rates. SAV versus the effects of LPS on AKT1, Fas, TNF-α and IFN-γ mRNA expression. SAV was found to significantly lower Fas gene expression comparing to the LPS group and restore the level of IFN-γ mRNA expression to that of the control group.ConclusionIn conclusion, SAV, at the dose of 100 μg/kg body weight, maintained and restored the oxidative stability, the anti-inflammatory, and the hypolipidemic bioactivity in rats after induced disruption of these parameters by LPS injection. This improvement by SAV was mediated by upregulation of AKT1.

【 授权许可】

CC BY   
© Ebaid et al.; licensee BioMed Central Ltd. 2012