Bioactivity of Samsum ant (Pachycondyla sennaarensis) venom against lipopolysaccharides through antioxidant and upregulation of Akt1 signaling in rats

Lipids in Health and Disease

Saad Gadoa³  Ahmed M Isa¹  Mohamed Al-Khalifa³  Hossam Ebaid² 

[1] Department of OB GYNE, College of Medicine, King Saud University, P.O.Box 7805, Riyadh, 11472, Saudi Arabia;Permanent address: Department of Zoology, Faculty of ScienceEl-Minia University, Minya, Egypt;Department of Zoology, College of Science, King Saud University, P.O.Box 2455, Riyadh, 11451, Saudi Arabia

关键词: Lipopolysaccharides;    Anti-inflammatory;    Oxidative stress;    Samsum ant venom;    AKT1;   

Others  :  1160245
DOI  :  10.1186/1476-511X-11-93

 received in 2012-04-24, accepted in 2012-07-03,  发布年份 2012

【 摘 要 】

Background

This study aimed at investigating the oxidative stress ameliorating effect, lipids profile restoration, and the anti-inflammatory effect of Samsum Ant Venom (SAV) in induced endotoxemic male rats, injected with bacterial lipopolysaccharides (LPS).

Results

Results revealed that LPS significantly increased the oxidative stress indications in LPS-injected rats. A significant increase of both malondialdehyde (MDA), and advanced oxidative protein products (AOPP), as well as a significant suppression of glutathione were all detected. Treatment with 100 μg/kg dose of SAV significantly restored the oxidative stress normal indications and increased the total glutathione levels. Treatment of the LPS-rats with 100 μg/kg dose of SAV showed a clear anti-inflammatory function; as the histological architecture of the hepatic tissue was partially recovered, along with a valuable decrease in the leukocytes infiltrated the hepatic tissues. Treatment of some rat groups with 600 μg/kg dose of SAV after LPS injection induced a severe endotoxemia that resulted in very high mortality rates. SAV versus the effects of LPS on AKT1, Fas, TNF-α and IFN-γ mRNA expression. SAV was found to significantly lower Fas gene expression comparing to the LPS group and restore the level of IFN-γ mRNA expression to that of the control group.

Conclusion

In conclusion, SAV, at the dose of 100 μg/kg body weight, maintained and restored the oxidative stability, the anti-inflammatory, and the hypolipidemic bioactivity in rats after induced disruption of these parameters by LPS injection. This improvement by SAV was mediated by upregulation of AKT1.

【 授权许可】

   
2012 Ebaid et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Bluthgen N, Stork NE, Fiedler K: Bottom-up control and co-occurrence in complex communities: honeydew and nectar determine a rainforest ant mosaic. Oikos 2004, 106:344-358.
• [2]Rifflet A, Tene N, Orivel J, Treilhou M, Dejean A, Vetillard A: Paralyzing action from a distance in an arboreal African ant species. PLoS One 2011, 6:e28571.
• [3]Marlier JF, Quinet Y, De Biseau JC: Defensive behaviour and biological activities of the abdominal secretion in the ant Crematogaster scutellaris (Hymenoptera: Myrmicinae). Behav Proc 2004, 67:427-440.
• [4]Pasteels JM, Daloze D, Boeve´ JL: Aldehydic contact poisons and alarm pheromone of the ant Crematogaster scutellaris (Hymenoptera: Myrmicinae). Enzyme-Mediated Production from acetate precursors. J Chem Ecol 1989, 15:1501-1511.
• [5]Leclercq S, de Biseau JC, Braekman JC, Daloze D, Quinet Y: Furanocembranoid diterpenes as defensive compounds in the Dufour gland of the ant Crematogaster brevispinosa rochaı. Tetrahedron 2000, 56:2037-2042.
• [6]Laurent P, Braekman JC, Daloze D: Insect chemical defense. Top Curr Chemist 2005, 240:167-229.
• [7]Wiese MD, Davies NW, Chataway TK, Milne RW, Brown SG, Heddle RJ: Stability of Myrmecia pilosula (Jack Jumper) Ant venom for use in immunotherapy. J Pharm Biomed Anal 2011, 54:303-310.
• [8]Collingwood CA: Hymenoptera: Fam. Formicidae of Saudi Arabia. Fauna. Saudi Arabia 1985, 7:230-302.
• [9]Al-Shahwan M, Al-Khenaizan S, Al-Khalifa M: Black (samsum) ant induced anaphylaxis in Saudi Arabia. Saudi med J 2006, 27:1761-1763.
• [10]Alsharani M, Alanazi M, Alsalamah M: Black ant stings caused by Pachycondyla sennaarensis: a significant health hazard. Ann Saudi Med 2009, 29:207-211.
• [11]Potier A, Lavigne C, Chappard D, Verret JL, Chevailler A, Nicolie B, Drouet M: Cutaneous manifestations in Hymenoptera and Diptera anaphylaxis: relationship with basal serum tryptase. Clin Exp Allergy 2009, 39:717-25.
• [12]Wang CP, Wu YL: Study on mechanism underlying the treatment of rheumatoid arthritis by Keshiling. Zhongguo Zhong Yao Za Zhi 2006, 31:155-8.
• [13]Kou J, Ni Y, Li N, Wang J, Liu L, Jiang ZH: Analgesic and anti-inflammatory activities of total extract and individual fractions of Chinese medicinal ants. Polyrhachis lamellidens. Biol Pharm Bull 2005, 28:176-80.
• [14]Dkhil M, Abdel-Baki A, Al-Quraishi S, Al-Khalifa M: Anti-inflammatory activity of the venom from samsumants Pachycondyla sennaarensis. Afr J Pharm Pharmacol 2010, 4:115-118.
• [15]Badr G, Garraud O, Daghestani M, Al-Khalifa MS, Richard Y: Human breast carcinoma cells are induced to apoptosis by samsum ant venom through an IGF-1-dependant pathway, PI3K/AKT and ERK signaling. Cell Immunol 2012, 273:6-10.
• [16]Nikbakhtzadeh MR, Tirgari S, Fakoorziba HA: Two volatiles from the venom gland of the Samsum ant, Pachycondyla Sennaarensis. Toxicon 2009, 54:80-82.
• [17]Clark KD, Lu Z, Strand MR: Regulation of melanization by glutathione in the moth Pseudoplusia includens. Insect Biochem Molecul Biol 2010, 40:460-467.
• [18]Okhawa H, Ohishi N, Yagi K: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979, 95:351-358.
• [19]Kayali R, Cakatay U, Akcay T, Altug T: Effect of alpha-lipoic acid supplementation on markers of protein oxidation in post-mitotic tissues of ageing rat. Cell Biochem Funct 2006, 24:79-85.
• [20]Allain CC, Poon LS, Chan CS, Richmond W, Fu PC: Enzymatic determination of total serum cholesterol. Clin Chem 1974, 20:470-475.
• [21]Crack Peter J, Taylor Juliet M: Reactive oxygen species and the modulation of stroke Review Article. Free Radic Biol Med 2005, 38:1433-1444.
• [22]Hoffman DJ: Early nutrition and adult health: Perspectives for international and community nutrition programs and policies. Nutr Res Pract 2010, 4:449-454.
• [23]Ebaid H, Ajarem J, Abu Taweel G: Acute Endotoxemia During Gestation Induces Organ Dysfunction and Tissue Damage in Mouse Offspring. Pakistan J Zool 2012, 44:765-776.
• [24]Ebaid H, Salem A, Sayed A, Metwalli A: Whey protein enhances normal inflammatory responses during cutaneous wound healing in diabetic rats. Lipids Health Dis 2011, 10:235. BioMed Central Full Text
• [25]Ebaid H, Mohamed AD, Danfour MA, Tohamy A, Gabry MS: Piroxicam-induced hepatic and renal histopathological changes in mice. Lybian J Medicine 2007, 2:1-16.
• [26]Scirocco A, Matarrese P, Petitta C, Cicenia A, Ascione B, Mannironi C, Ammoscato F, Cardi M, Fanello G, Guarino MP, Malorni W, Severi C: Exposure of Toll-like receptors 4 to bacterial lipopolysaccharide (LPS) impairs human colonic smooth muscle cell function. J Cell Physiol 2010, 223(2):442-50.
• [27]Yazar E, Er A, Uney K, Bulbul A, Avci GE, Elmas M, Tras B: Effects of drugs used in endotoxic shock on oxidative stress and organ damage markers. Free Radic Res 2010, 44:397-402.
• [28]Golbidi S, Laher I: Med Sci Monit. 2010, 16(1):RA9-RA24.
• [29]Kaufmann GF, Sartorio R, Lee SH: Revisiting quorum sensing: discovery of additional chemical and biological functions for 3-oxo-Nacylhomoserine lactones. Proc Natl Acad Sci USA 2005, 102:309-314.
• [30]Orivel J, Redeker V, Le Caer JP, Krier F, Revol-Junelles AM, Longeon A, Chaffotte A, Dejean A, Rossier J, Ponericins : New antibacterial and insecticidal peptides from the venomof the ant Pachycondyla goeldii. J Biol Chem 2001, 76:17823-17829.
• [31]Lim Y, Levy M, Bray TM: Dietary zinc alters early inflammatory responses during cutaneous wound healing in weanling CD-1 mice. J Nutr 2004, 134:811-816.
• [32]Cheh N, Chipitsyna G, Gong Q, Yeo CJ, Arafat HA: Anti-inflammatory effects of the Nigella sativa seed extract, thymoquinone, in pancreatic cancer cells. HPB (Oxford) 2009, 11:373-381.
• [33]Kowalski J, Blada P, Kucia K, Madej A, Herman Z: Neuroleptics normalize increased release of interleukin- 1 beta and tumor necrosis factoralpha from monocytes in schizophrenia. Schizophr Res 2001, 50:169-175.
• [34]Kotb M, Calandra T: Cytokines and Chemokines in Infectious Diseases Handbook. New Jersey: Humana Press Totowa; 2003.
• [35]Nagata S: Apoptosis by death factor. Cell 1997, 88:355-365.
• [36]Takemura Y, Keisuke F, Osamu Y, Takahito I, Norio I, Toyohiko Y, Hidenobu K, Toshimitsu S, Toshio O: Fas Signaling Induces Akt Activation and Upregulation of Endothelial Nitric Oxide Synthase Expression. Hypertension 2004, 43:880-884.
• [37]Ezell SA, Polytarchou C, Hatziapostolou M, Guo A, Sanidas I, Bihani T, Comb MJ, Sourvinos G, Tsichlis PN: The protein kinase Akt1 regulates the interferon response through phosphorylation of the transcriptional repressor EMSY. Proc Natl Acad Sci USA 2012, 109:E613-21.
• [38]Kaur S, Sassano A, Dolniak B, Joshi S, Majchrzak-Kita B, Baker DP, Hay N, Fish EN, Platanias LC: Role of the Akt pathway in mRNA translation of interferon-stimulated genes. Proc Natl Acad Sci USA 2008, 105:4808-4813.
• [39]Kane LP, Andres PG, Howland KC, Abbas AK, Weiss A: Akt provides the CD28 costimulatory signal for up-regulation of IL-2 and IFN-gamma but not TH2 cytokines. Nat Immunol 2001, 2:37-44.
• [40]Kauffmann-Zeh A, Rodriguez-Viciana P, Ulrich E, Gilbert C, Coffer P, Downward J, Evan G: Suppression of c-Myc-induced apoptosis by Ras signalling through PI(3)K and PKB. Nature 1997, 385:544-548.
• [41]Engedal N: Immune regulator vitamin A and T cell death. Vitam Horm 2011, 86:153-178.
• [42]Gomez TS, Hamann MJ, McCarney S, Savoy DN, Lubking CM, Heldebrant MP, Labno CM, McKean DJ, McNiven MA, Burkhardt JK, Billadeau DD: Dynamin 2 regulates T cell activation by controlling actin polymerization at the immunological synapse. Nat Immunol 2005, 6:261-270.
• [43]Fuse S, Zhang W, Usherwood EJ: Control of memory CD8+ T cell differentiation by CD80/CD86-CD28 costimulation and restoration by IL-2 during the recall response. J Immunol 2008, 180:1148-1157.