A shift in the virulence potential of Corynebacterium pseudotuberculosis biovar ovis after passage in a murine host demonstrated through comparative proteomics

BMC Microbiology

Researc Article

Henrique Figueiredo¹  Vasco Azevedo²  Núbia Seyffert²  Fernanda A. Dorella²  Siomar C. Soares²  Thiago L. P. Castro²  Anderson Miyoshi²  Wanderson M. Silva³  Yves Le Loir⁴  Artur Silva⁵  Gustavo H. M. F. Souza⁶ 

[1]Aquacen, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

[2]Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

[3]Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

[4]INRA, UMR1253 STLO, 35042, Rennes, France

[5]Agrocampus Ouest, UMR1253 STLO, 35042, Rennes, France

[6]INRA, UMR1253 STLO, 35042, Rennes, France

[7]Agrocampus Ouest, UMR1253 STLO, 35042, Rennes, France

[8]Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, Belém, Pará, Brazil

[9]Waters Corporation, Waters Technologies Brazil, MS Applications Laboratory, Alphaville, São Paulo, Brazil

关键词: Corynebacterium pseudotuberculosis;    Bacterial label-free proteomic;    Caseous lymphadenitis;    Bacterial virulence;    Serial passage;    Extracellular proteins;   

DOI  :  10.1186/s12866-017-0925-6

 received in 2016-01-15, accepted in 2017-01-04,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundCorynebacterium pseudotuberculosis biovar ovis, a facultative intracellular pathogen, is the etiologic agent of caseous lymphadenitis in small ruminants. During the infection process, C. pseudotuberculosis changes its gene expression to resist different types of stresses and to evade the immune system of the host. However, factors contributing to the infectious process of this pathogen are still poorly documented. To better understand the C. pseudotuberculosis infection process and to identify potential factors which could be involved in its virulence, experimental infection was carried out in a murine model using the strain 1002_ovis and followed by a comparative proteomic analysis of the strain before and after passage.ResultsThe experimental infection assays revealed that strain 1002_ovis exhibits low virulence potential. However, the strain recovered from the spleen of infected mice and used in a new infection challenge showed a dramatic change in its virulence potential. Label-free proteomic analysis of the culture supernatants of strain 1002_ovis before and after passage in mice revealed that 118 proteins were differentially expressed. The proteome exclusive to the recovered strain contained important virulence factors such as CP40 proteinase and phospholipase D exotoxin, the major virulence factor of C. pseudotuberculosis. Also, the proteome from recovered condition revealed different classes of proteins involved in detoxification processes, pathogenesis and export pathways, indicating the presence of distinct mechanisms that could contribute in the infectious process of this pathogen.ConclusionsThis study shows that C. pseudotuberculosis modifies its proteomic profile in the laboratory versus infection conditions and adapts to the host context during the infection process. The screening proteomic performed us enable identify known virulence factors, as well as potential proteins that could be related to virulence this pathogen. These results enhance our understanding of the factors that might influence in the virulence of C. pseudotuberculosis.

【 授权许可】

CC BY   
© The Author(s). 2017