MicroRNA expression patterns in post-natal mouse skeletal muscle development

BMC Genomics

Research Article

Liliana Orellana¹  Séverine Lamon²  Jasmine Mikovic²  Aaron P. Russell²  Evelyn Zacharewicz²  Miranda D. Grounds³  Lauren C. Butchart³ 

[1] Deakin University, Biostatistics Unit, Faculty of Health, Geelong, Australia;Deakin University, School of Exercise and Nutrition Sciences, Institute for Physical Activity and Nutrition (I-PAN), Geelong, Australia;The University of Western Australia, School of Anatomy, Physiology and Human Biology, Perth, WA, Australia;

关键词: MiRNAs;    Skeletal muscle;    Myogenesis;    Growth;    Cell differentiation;    Cell proliferation;   

DOI  :  10.1186/s12864-016-3399-2

 received in 2016-06-30, accepted in 2016-12-08,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMiRNAs are essential regulators of skeletal muscle development and homeostasis. To date, the role and regulation of miRNAs in myogenesis have been mostly studied in tissue culture and during embryogenesis. However, little information relating to miRNA regulation during early post-natal skeletal muscle growth in mammals is available. Using a high-throughput miRNA qPCR-based array, followed by stringent statistical and bioinformatics analysis, we describe the expression pattern and putative role of 768 miRNAs in the quadriceps muscle of mice aged 2 days, 2 weeks, 4 weeks and 12 weeks.ResultsForty-six percent of all measured miRNAs were expressed in mouse quadriceps muscle during the first 12 weeks of life. We report unprecedented changes in miRNA expression levels over time. The expression of a majority of miRNAs significantly decreased with post-natal muscle maturation in vivo. MiRNA clustering identified 2 subsets of miRNAs that are potentially involved in cell proliferation and differentiation, mainly via the regulation of non-muscle specific targets.ConclusionCollective miRNA expression in mouse quadriceps muscle is subjected to substantial levels of regulation during the first 12 weeks of age. This study identified a new suite of highly conserved miRNAs that are predicted to influence early muscle development. As such it provides novel knowledge pertaining to post-natal myogenesis and muscle regeneration in mammals.

【 授权许可】

CC BY   
© The Author(s). 2017