Engineering Escherichia coli K12 MG1655 to use starch

Microbial Cell Factories

Research

Agustino Martínez-Antonio¹  Luis Manuel Rosales-Colunga² 

[1] Departamento de Ingeniería Genética, CINVESTAV, Km 9.6 Libramiento Norte Carr. Irapuato-León, Gto, 36821, Irapuato, México;Departamento de Ingeniería Genética, CINVESTAV, Km 9.6 Libramiento Norte Carr. Irapuato-León, Gto, 36821, Irapuato, México;Facultad de Ingeniería, Universidad Autónoma de San Luis Potosí, Av. Dr.Manuel Nava 8, Zona Universitaria, San Luis Potosí, SLP, México;

关键词: Amylase;    Synthetic biology;    Adaptive strain;    Maltodextrins;    Bacteria;    E. coli;   

DOI  :  10.1186/1475-2859-13-74

 received in 2013-09-30, accepted in 2014-05-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundTo attain a sustainable bioeconomy, fuel, or valuable product, production must use biomass as substrate. Starch is one of the most abundant biomass resources and is present as waste or as a food and agroindustry by-product. Unfortunately, Escherichia coli, one of the most widely used microorganisms in biotechnological processes, cannot use starch as a carbon source.ResultsWe engineered an E. coli strain capable of using starch as a substrate. The genetic design employed the native capability of the bacterium to use maltodextrins as a carbon source plus expression and secretion of its endogenous α-amylase, AmyA, in an adapted background. Biomass production improved using 35% dissolved oxygen and pH 7.2 in a controlled bioreactor.ConclusionThe engineered E. coli strain can use starch from the milieu and open the possibility of optimize the process to use agroindustrial wastes to produce biofuels and other valuable chemicals.

【 授权许可】

CC BY   
© Rosales-Colunga and Martínez-Antonio; licensee BioMed Central Ltd. 2014