Genetic characterization of antimicrobial resistance of Shigella flexneri 1c isolates from patients in Egypt and Pakistan

Annals of Clinical Microbiology and Antimicrobials

Short Report

Amel Naguib¹  Jesse Monestersky²  John Klena²  Momtaz O Wasfy²  Salwa F Ahmed²  Tupur Husain² 

[1] Department of Epidemiology, Ministry of Health and Population, Cairo, Egypt;Research Science Directorate, United States Naval Medical Research Unit No. 3, Cairo, Egypt;

关键词: S. flexneri;    Genetic diversity;    Antibiotic resistance;    Oxa1;    PFGE;    Egypt;    Diarrhea;    Pakistan;   

DOI  :  10.1186/1476-0711-12-9

 received in 2013-02-06, accepted in 2013-04-14,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundShigella flexneri serotype 1c emerged as a critical isolate from children in Egypt and Pakistan. The pattern of antimicrobial susceptibility (AMS) and resistance genes of this serotype have yet to be characterized.FindingsSixty nine S. flexneri 1c isolates isolates were identified from both Egypt (n-46) and Pakistan (n = 23) and tested for AMS by disk diffusion method and minimal inhibitory concentrations were also determined. Isolates were genotyped by pulsed field gel electrophoresis (PFGE) and five relevant resistance genes (blaTEM, blaSHV, blaOXA, sul I and sul II) were detected by polymerase chain reaction (PCR) and confirmed by DNA sequencing. High resistance was observed in all isolates for ampicillin (AM >96%); trimethoprim-sulphamethoxazole and tetracycline (>88%). Most AM-resistant isolates from Egypt (70%) harbored blaTEM resistance, while 52% of isolates from Pakistan expressed blaOXA. All isolates were closely related by PFGE, irrespective of source or time of collection. The sul II gene was present in 100% of isolates from pediatric cases in Egypt, 65% of Pakistan isolates, and 53% of isolates from older Egyptian patients.ConclusionsWhile different Shigella serotypes gathered in specific genotypic groups, 1c serotype isolates formed multiple clusters. Although AMS was considerably high to most commonly used drugs, genetic determinants were variable between countries over time. The data stress the need for a more careful selection of antibiotics in the treatment of shigellosis.

【 授权许可】

CC BY   
© Ahmed et al.; licensee BioMed Central Ltd. 2013