Annals of Clinical Microbiology and Antimicrobials | |
Antibacterial and resistance-modifying activities of thymoquinone against oral pathogens | |
Research | |
Kamel Chaieb1  Tarek Zmantar1  Amina Bakhrouf1  Bochra Kouidhi1  Yosra Souiden2  Kacem Mahdouani2  Hanene Jrah3  | |
[1] Laboratory of Analyses, Treatments and Valorisation of Environmental Wastes and Products, Faculty of Pharmacy, Monastir University, Avicenne Street, 5000, Monastir, Tunisia;Laboratory of Molecular Biology, Kairouan Hospital, Tunisia;Research Unit of Biology and Genetics of Hematological and Auto-immune Diseases, Faculty of Pharmacy, Monastir University, Avicenne Street, 5000, Monastir, Tunisia; | |
关键词: Minimum Inhibitory Concentration; Tetracycline; Infective Endocarditis; Dental Caries; Propolis; | |
DOI : 10.1186/1476-0711-10-29 | |
received in 2011-04-04, accepted in 2011-06-27, 发布年份 2011 | |
来源: Springer | |
【 摘 要 】
BackgroundThe presence of resistant bacteria in the oral cavity can be the major cause of dental antibiotic prophylaxis failure. Multidrug efflux has been described for many organisms, including bacteria and fungi as part of their drugs resistance strategy. The discovery of a new efflux pump inhibitor could extend the useful lifetime of some antibiotics.MethodsIn this study, the MICs of thymoquinone (TQ), tetracycline and benzalkonium chloride (BC) were determined in absence and in presence of a sub-MIC doses of thymoquinone (1/2 MIC). In addition the 4,6-diamidino-2-phenylindole (DAPI) efflux assay was carried out to determine the effect of TQ on DAPI cells accumulation.ResultsTQ induced a selective antimicrobial activity. Its synergic effect resulted in at least a 4-fold potentiation of the tested antibiotics and antiseptic. In addition, TQ inhibited the DAPI efflux activity in a concentration-dependent manner. The rate of DAPI accumulation in clinical isolates was enhanced with TQ (0 to 200 μg/ml). There is also a decrease in loss of DAPI from bacteria in the presence of TQ. The concentration causing 50% of DAPI efflux inhibition after 15 minutes was approximately 59 μg/ml for Pseudomonas aeroginosa and 100 μg/ml and Staphylococcus aureus respectively.ConclusionsTQ possesses a selective antibacterial activity against oral bacteria. It is therefore suggested that TQ could be used as a source of natural products with resistance-modifying activity. Further investigation is needed to assess their clinical relevance.
【 授权许可】
CC BY
© Kouidhi et al; licensee BioMed Central Ltd. 2011
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202311107614236ZK.pdf | 759KB | download |
【 参考文献 】
- [1]
- [2]
- [3]
- [4]
- [5]
- [6]
- [7]
- [8]
- [9]
- [10]
- [11]
- [12]
- [13]
- [14]
- [15]
- [16]
- [17]
- [18]
- [19]
- [20]
- [21]
- [22]
- [23]
- [24]
- [25]
- [26]
- [27]
- [28]
- [29]
- [30]
- [31]
- [32]
- [33]
- [34]
- [35]