The antimicrobial resistance patterns and associated determinants in Streptococcus suisisolated from humans in southern Vietnam, 1997-2008

BMC Infectious Diseases

Research Article

Nguyen TH Mai¹  Nguyen VV Chau¹  Tran T Hien¹  Pham H Anh²  Stephen Baker²  Tran TB Chieu²  Ngo T Hoa²  Jeremy Farrar²  Marcel Wolbers²  James I Campbell²  Constance Schultsz³  Ho DT Nghia⁴ 

[1] Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam;Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam;Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam;Academic Medical Centre - Center for Poverty-related Communicable Diseases, University of Amsterdam, Amsterdam, The Netherlands;Pham Ngoc Thach Medical University, Ho Chi Minh City, Vietnam;

关键词: Minimum Inhibitory Concentration;    Tetracycline;    Resistance Determinant;    Tetracycline Resistance;    Erythromycin Resistance;   

DOI  :  10.1186/1471-2334-11-6

 received in 2010-04-21, accepted in 2011-01-06,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundStreptococcus suis is an emerging zoonotic pathogen and is the leading cause of bacterial meningitis in adults in Vietnam. Systematic data on the antimicrobial susceptibility profiles of S. suis strains isolated from human cases are lacking. We studied antimicrobial resistance and associated resistance determinants in S. suis isolated from patients with meningitis in southern Vietnam.MethodsS. suis strains isolated between 1997 and 2008 were investigated for their susceptibility to six antimicrobial agents. Strains were screened for the presence and expression of tetracycline and erythromycin resistance determinants and the association of tet(M) genes with Tn916- like transposons. The localization of tetracycline resistance gene tet(L) was determined by pulse field gel electrophoresis and Southern blotting.ResultsWe observed a significant increase in resistance to tetracycline and chloramphenicol, which was concurrent with an increase in multi-drug resistance. In tetracycline resistance strains, we identified tet(M), tet(O), tet(W) and tet(L) and confirmed their expression. All tet(M) genes were associated with a Tn916-like transposon. The co-expression of tet(L) and other tetracycline resistance gene(s) encoding for ribosomal protection protein(s) was only detected in strains with a minimum inhibitory concentration (MIC) of tetracycline of ≥ 64 mg/LConclusionsWe demonstrated that multi-drug resistance in S. suis causing disease in humans in southern Vietnam has increased over the 11-year period studied. We report the presence and expression of tet(L) in S. suis strains and our data suggest that co-expression of multiple genes encoding distinct mechanism is required for an MIC ≥ 64 mg/L to tetracycline.

【 授权许可】

CC BY   
© Hoa et al; licensee BioMed Central Ltd. 2011