Behaviour and molecular identification of Anopheles malaria vectors in Jayapura district, Papua province, Indonesia

Malaria Journal

Research

Asik Surya¹  Frank H. Collins²  Neil F. Lobo²  John Mueller²  David Bretz²  Michelle Ngai²  Brandy St. Laurent²  Helen Catherine Miller²  Puji Budi Setia Asih³  Din Syafruddin⁴  Sukowati Supratman⁵  Ferdinand Laihad⁶  William A. Hawley⁷  Amirullah Baharuddin⁸ 

[1] Department of Parasitology, Faculty of Medicine, Hasanuddin University, 90245, Makasaar, Indonesia;Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN, USA;Eijkman Institute for Molecular Biology, Jakarta, Indonesia;Eijkman Institute for Molecular Biology, Jakarta, Indonesia;Department of Parasitology, Faculty of Medicine, Hasanuddin University, 90245, Makasaar, Indonesia;National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia;National Malaria Control Programme, Ministry of Health, Jakarta, Indonesia;UNICEF, Jakarta, Indonesia;University of Halueleo, Kendari, Indonesia;

关键词: Malaria;    Anopheles;    Vector ecology;    Molecular tools;    Indonesia;   

DOI  :  10.1186/s12936-016-1234-5

 received in 2015-12-09, accepted in 2016-03-15,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMembers of the Anopheles punctulatus group dominate Papua, Indonesia and Papua New Guinea (PNG), with a geographic range that extends south through Vanuatu. An. farauti and An. punctulatus are the presumed major vectors in this region. Although this group of species has been extensively studied in PNG and the southern archipelagoes within their range, their distribution, ecology and vector behaviours have not been well characterized in eastern Indonesia.MethodsMosquitoes were collected in five villages in Jayapura province, Papua, Indonesia using human-landing collections, animal-baited tents and backpack aspirators. Mosquitoes were morphologically typed and then molecularly distinguished based on ribosomal ITS2 sequences and tested for Plasmodium falciparum and P. vivax infection using circumsporozoite ELISA and PCR.ResultsThe presence and vector status of An. farauti4 in Papua, Indonesia is confirmed here for the first time. The data indicate that this species is entering houses at a rate that increases its potential to come into contact with humans and act as a major malaria vector. An. farauti 4 was also abundant outdoors and biting humans during early evening hours. Other species collected in this area include An. farauti 1, An. hinesorum, An. koliensis, An. punctulatus, and An. tessellatus. Proboscis morphology was highly variable within each species, lending support to the notion that this characteristic is not a reliable indicator to distinguish species within the An. punctulatus group.ConclusionsThe vector composition in Papua, Indonesia is consistent with certain northern areas of PNG, but the behaviours of anophelines sampled in this region, such as early and indoor human biting of An. farauti 4, may enable them to act as major vectors of malaria. Presumed major vectors An. farauti and An. punctulatus were not abundant among these samples. Morphological identification of anophelines in this sample was often inaccurate, highlighting the importance of using molecular analysis in conjunction with morphological investigations to update keys and training tools.

【 授权许可】

CC BY   
© St. Laurent et al. 2016