Host attraction and biting behaviour of Anopheles mosquitoes in South Halmahera, Indonesia

Malaria Journal

Research

Thomas R. Burkot¹  William A. Hawley²  Frank H. Collins³  Neil F. Lobo³  Timothy A. Burton³  Helen C. Miller³  Brandyce St. Laurent⁴  Siti Zubaidah⁵  Din Syafruddin⁵  Sully Kosasih⁵  Puji B. Asih⁵  Supratman Sukowati⁶  Saya Firman⁶  Amirullah Baharuddin⁶ 

[1] Centers for Disease Control and Prevention, Atlanta, GA, USA;Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, Australia;Centers for Disease Control and Prevention, Atlanta, GA, USA;Unicef, Jakarta, Indonesia;Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN, USA;Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN, USA;Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA;Eijkman Institute for Molecular Biology, Jakarta, Indonesia;National Institute of Health Research and Development, Jakarta, Indonesia;

关键词: Anopheles;    Indonesia;    Malaria;    Biting behavior;    Host attraction;    Vector ecology;   

DOI  :  10.1186/s12936-017-1950-5

 received in 2017-05-02, accepted in 2017-07-20,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundIndonesia is home to a variety of malaria vectors whose specific bionomic traits remain largely uncharacterized. Species-specific behaviours, such as host feeding preferences, impact the dynamics of malaria transmission and the effectiveness of vector control interventions.MethodsTo examine species-specific host attraction and feeding behaviours, a Latin square design was used to compare Anopheles mosquitoes attracted to human, cow, and goat-baited tents. Anopheles mosquitoes were collected hourly from the inside walls of each baited tent. Species were morphologically and then molecularly identified using rDNA ITS2 sequences. The head and thorax of individual specimens were analysed for Plasmodium DNA using PCR. Bloodmeals were identified using a multiplex PCR.ResultsA total of 1024, 137, and 74 Anopheles were collected over 12 nights in cow, goat, and human-baited tents, respectively. The species were identified as Anopheles kochi, Anopheles farauti s.s., Anopheles hackeri, Anopheles hinesorum, Anopheles indefinitus, Anopheles punctulatus, Anopheles tessellatus, Anopheles vagus, and Anopheles vanus, many of which are known to transmit human malaria. Molecular analysis of blood meals revealed a high level of feeding on multiple host species in a single night. Anopheles kochi, An. indefinitus, and An. vanus were infected with Plasmodium vivax at rates comparable to primary malaria vectors.ConclusionsThe species distributions of Anopheles mosquitoes attracted to human, goat, and cow hosts were similar. Eight of nine sporozoite positive samples were captured with animal-baited traps, indicating that even predominantly zoophilic mosquitoes may be contributing to malaria transmission. Multiple host feeding and flexibility in blood feeding behaviour have important implications for malaria transmission, malaria control, and the effectiveness of intervention and monitoring methods, particularly those that target human-feeding vectors.

【 授权许可】

CC BY   
© The Author(s) 2017