Cytoadherence phenotype of Plasmodium falciparum- infected erythrocytes is associated with specific pfemp-1 expression in parasites from children with cerebral malaria

Malaria Journal

Research

Sem Ezimegnon¹  Charles Ahouansou¹  Maroufou J Alao²  Philippe Deloron³  Talleh Almelli³  Rachida Tahar³  Nicaise T Ndam³  Gratien Sagbo⁴  Annick Amoussou⁵ 

[1] Centre d’Etude et de recherche sur le Paludisme Associé à la Grossesse et l’Enfance (CERPAGE), Cotonou, Bénin;Département de pédiatrie, Hôpital Mère-enfant de la lagune (HOMEL), Cotonou, Bénin;Institut de Recherche pour le Développement (IRD), UMR 216 Mère et Enfant Face aux Infections Tropicales, 4, avenue de l’Observatoire, 75270, Paris, France;PRES Sorbonne Paris Cité, Université Paris Descartes, Faculté de Pharmacie, Paris, France;Service de pédiatrie, Centre National Hospitalo-Universitaire (CNHU), Cotonou, Bénin;Service de pédiatrie, Hôpital de zone de Suru-Léré, Cotonou, Bénin;

关键词: Plasmodium falciparum;    Cerebral malaria;    Uncomplicated malaria;    Var;    Domain cassette;    Transcript abundance;    Cytoadherence;    CD36;    ICAM-1;    CSPG;    CSA;   

DOI  :  10.1186/1475-2875-13-333

 received in 2014-05-29, accepted in 2014-07-29,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundCytoadherence of Plasmodium falciparum- infected erythrocytes (IEs) in deep microvasculature endothelia plays a major role in the pathogenesis of cerebral malaria (CM). This biological process is thought to be mediated by P. falciparum erythrocyte membrane protein-1 (PfEMP-1) and human receptors such as CD36 and ICAM-1. The relationship between the expression of PfEMP-1 variants and cytoadherence phenotype in the pathology of malaria is not well established.MethodsCytoadherence phenotypes of IEs to CD36, ICAM-1, CSPG and the transcription patterns of A, B, var2csa, var3, var gene groups and domain cassettes DC8 and DC13 were assessed in parasites from children with CM and uncomplicated malaria (UM) to determine if cytoadherence is related to a specific transcription profile of pfemp-1 variants.ResultsParasites from CM patients bind significantly more to CD36 than those from UM patients, but no difference was observed in their binding ability to ICAM-1 and CSPG. CM isolates highly transcribed groups A, B, var2csa, var3, DC8 and DC13 compared to UM parasites. The high transcription levels of var genes belonging to group B positively correlated with increased binding level to CD36.ConclusionCM isolates bind significantly more to CD36 than to ICAM-1, which was correlated with high transcription level of group B var genes, supporting their implication in malaria pathogenesis.

【 授权许可】

Unknown   
© Almelli et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.