The regulation of toll-like receptor 2 by miR-143 suppresses the invasion and migration of a subset of human colorectal carcinoma cells

Molecular Cancer

Research

Guanxiang Qian¹  Haiyan Guo²  Xiaobo Hu²  Shengfang Ge³  Jianjun Zhang⁴  Ying Chen⁵ 

[1] Department of Biochemistry and Molecular Biology, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China;Department of Clinical Laboratory, Shanghai Third People’s Hospital, School of medicine, Shanghai Jiao Tong University, 200019, Shanghai, PR China;Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China;Department of Biochemistry and Molecular Biology, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China;Department of Oral & Maxillofacial-Head Neck Oncology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 200011, Shanghai, PR China;Department of Oral & Maxillofacial-Head Neck Oncology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 200011, Shanghai, PR China;Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China;

关键词: miR-143;    Toll-like receptor 2;    Colorectal carcinoma;    Invasion;    Migration;   

DOI  :  10.1186/1476-4598-12-77

 received in 2013-04-11, accepted in 2013-07-12,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe Toll-like receptor 2 (TLR2)-driven tissue response may promote neoangiogenesis and tumour growth by mechanisms that are poorly understood.MethodsWe investigated the expression levels of TLR2 and associated-miRNAs in colorectal carcinoma (CRC) tissues and cell lines using real-time PCR, northern blotting and western blotting. Survival curver was generated by Log-Rank test and the role of TLR2 signalling in tumour invasion and migration was determined by transwell analysis kits.ResultsWe observed that the tissues from CRC patients express relatively high levels of TLR2. Targeting TLR2 markedly reduces the invasion and migration of CRC cells. We also found that miR-143, a putative tumour suppressor that is down-regulated in CRC tissues, reduces the invasion and migration of CRC cells primarily via TLR2. Utilising a xenograft mouse model, we demonstrated that re-expression of miR-143 inhibits CRC cell colonisation in vivo.ConclusionmiR-143 blocks the TLR2 signalling pathway in human CRC cells. This knowledge may pave the way for new clinical applications utilising miR-143 mimics in the treatment of patients with CRC.

【 授权许可】

CC BY   
© Guo et al.; licensee BioMed Central Ltd. 2013