Journal of Biomedical Science | |
Neuroprotective peptide ADNF-9 in fetal brain of C57BL/6 mice exposed prenatally to alcohol | |
Research | |
Ahmed YoussefAgha1  Zaneer M Segu2  Jonathan A Karty2  Dragan Isailovic3  Youssef Sari4  | |
[1] Department of Applied Health Science, Indiana University, Bloomington, IN, US;Department of Chemistry, Indiana University, Bloomington, IN, US;Department of Chemistry, University of Toledo, Toledo, OH, US;Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, OH, US; | |
关键词: Fetal Brain; Alcohol Exposure; Liquid Diet; Fetal Alcohol Syndrome; Prenatal Alcohol Exposure; | |
DOI : 10.1186/1423-0127-18-77 | |
received in 2011-08-24, accepted in 2011-10-21, 发布年份 2011 | |
来源: Springer | |
【 摘 要 】
BackgroundA derived peptide from activity-dependent neurotrophic factor (ADNF-9) has been shown to be neuroprotective in the fetal alcohol exposure model. We investigated the neuroprotective effects of ADNF-9 against alcohol-induced apoptosis using TUNEL staining. We further characterize in this study the proteomic architecture underlying the role of ADNF-9 against ethanol teratogenesis during early fetal brain development using liquid chromatography in conjunction with tandem mass spectrometry (LC-MS/MS).MethodsPregnant C57BL/6 mice were exposed from embryonic days 7-13 (E7-E13) to a 25% ethanol-derived calorie [25% EDC, Alcohol (ALC)] diet, a 25% EDC diet simultaneously administered i.p. ADNF-9 (ALC/ADNF-9), or a pair-fed (PF) liquid diet. At E13, fetal brains were collected from 5 dams from each group, weighed, and frozen for LC-MS/MS procedure. Other fetal brains were fixed for TUNEL staining.ResultsAdministration of ADNF-9 prevented alcohol-induced reduction in fetal brain weight and alcohol-induced increases in cell death. Moreover, individual fetal brains were analyzed by LC-MS/MS. Statistical differences in the amounts of proteins between the ALC and ALC/ADNF-9 groups resulted in a distinct data-clustering. Significant upregulation of several important proteins involved in brain development were found in the ALC/ADNF-9 group as compared to the ALC group.ConclusionThese findings provide information on potential mechanisms underlying the neuroprotective effects of ADNF-9 in the fetal alcohol exposure model.
【 授权许可】
CC BY
© Sari et al; licensee BioMed Central Ltd. 2011
【 预 览 】
Files | Size | Format | View |
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RO202311107398784ZK.pdf | 737KB | download |
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