Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium

BMC Gastroenterology

Research Article

Sinforiano J Posadas¹  Marcos Elvira¹  Isabel Caballero¹  Victor Caz¹  Enrique De Miguel¹  Carlota Largo¹  Immaculada Quilez²  Emilio Cendejas² 

[1] Experimental Surgery Department, La Paz Hospital, Paseo de la Castellana 261, 28046, Madrid, Spain;Microbiology Department, La Paz Hospital, Paseo de la Castellana 261, 28046, Madrid, Spain;

关键词: TLR5 Expression;    Liquid Diet;    Yeast Cell Wall;    TLR5 mRNA Expression;    Saccharomyces Boulardii;   

DOI  :  10.1186/1471-230X-10-58

 received in 2009-06-16, accepted in 2010-06-08,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundMannoproteins are yeast cell wall componend, and rich in mannose. The use of foods rich in mannose as carbohydrate, could have a bioprotective effect against entrobacteria intestinal infection. Nothing is known about mannoproteins' activity in inflammatory bowel processes induced by entrobacteria.This study investigates the effects of mannoprotein administration via a liquid diet on inflammatory response and TLR5 expression during intestinal tissue injury in a rat model of infection with Salmonella typhimurium.MethodsAdult Wistar male rats were divided into three groups: control, and mannoprotein E1 at 10 or 15%. Animals were fed with a liquid diet supplemented or not with mannoprotein E1. Groups were infected by intragastrical administration of S. typhimurium. 24 h post-inoculation samples of spleen, ileum and liver were collected for microbiological studies. Gut samples were processed to determine levels of proinflammatory cytokines (mRNA) and TLR5 (mRNA and protein) by quantitative PCR and Western-blot, and the number of proliferative and apoptotic cells determined by immunohistochemistry.ResultsIninfected levels of proinflammatory cytokines and TLR5 were higher in untreated controls than in the animals receiving mannoprotein. Proliferation was similar in both groups, whereas apoptosis was higher in controls. Curiosly, the mannoprotein effect was dose dependent.ConclusionsMannoprotein administration in a liquid diet seems to protect intestinal tissue against S. typhimurium infection. This protection seems to expressed as a lower pro-inflammatory response and TLR5 downregulation in gut epithelium, as well as by an inhibition of apoptosis. Nevertheless, the molecular mechanism by which mannoprotein is able to regulate these responses remain unclear. These results could open up new avenues in the use of mannoproteins as prebiotics in the therapeutic strategy for treatment of inflammatory gut processes induced by microbia.

【 授权许可】

Unknown   
© Posadas et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.