Expression of chemokine receptors on circulating tumor cells in patients with solid tumors

Journal of Translational Medicine

Research

Alberto Fusi¹  Anika Nonnemacher¹  Ulrich Keilholz¹  Zhian Liu¹  Verena Kümmerlen¹  Judith Jeske¹ 

[1] Department of Hematology and Medical Oncology, Charité, Campus Benjamin Franklin, Hindenburgdamm 30, 12200, Berlin, Germany;

关键词: Circulating tumor cells;    Chemokine receptor;    CD45 depletion;    CXCR4;    CCR6;    CCR7;    CCR9;   

DOI  :  10.1186/1479-5876-10-52

 received in 2011-10-18, accepted in 2012-03-20,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe study was performed to investigate the expression of chemokine receptors (CR) on circulating tumor cells (CTC), which may be of importance for organ-specific metastases and cancer treatment since CR are potential drug-targets.MethodsBlood samples from patients with metastatic carcinoma (MC) or melanoma (MM) were enriched for CTC and expression of CR (CXCR4, CCR6, CCR7 and CCR9) was evaluated by flow cytometry.ResultsCTC were detected in 49 of 68 patients (72%) [28 MC; 21 MM] with a median number of 3 CTC (range: 1-94)/10 mL of blood. CXCR4 was expressed on CTC in 82% (40/49) of patients [median number 1 CTC/10 mL blood; range 1-14] and CCR6 in 29 patients (59%; median 1, range: 1-14). In MM patients, CCR7 was expressed on CTC in 6 (29%) samples and CCR9 in 12 (57%). A positive correlation between surface expression of CR and organ-specific metastatic pattern was not observed.ConclusionsCR were expressed on CTC of patients with solid tumors. Along with our findings, the observation that CR could be involved in CTC proliferation and migration of tumor cells appoints CTC as potential CR-antagonist therapeutic target.

【 授权许可】

Unknown   
© Fusi et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.