In vitro antifungal activity of hydroxychavicol isolated from Piper betle L

Annals of Clinical Microbiology and Antimicrobials

Research

Inshad A Khan¹  Intzar Ali¹  Ghulam N Qazi²  Farhat Afrin²  Farrah G Khan³  Prabhu Dutt⁴  Naresh K Satti⁴  Bishan D Gupta⁴  Krishan A Suri⁴ 

[1] Clinical Microbiology Division Indian Institute of Integrative Medicine, Canal Road, 180 001, Jammu, India;Department of Biotechnology, Faculty of Science, Hamdard University, Hamdard Nagar, 110 062, New Delhi, India;Department of Microbiology, Acharya Shri Chander College of Medical Sciences, Sidhra, 180 017, Jammu, India;Natural Product Chemistry Division, Indian Institute of Integrative Medicine, Canal Road, 180 001, Jammu, India;

关键词: Minimum Inhibitory Concentration;    Propidium Iodide;    Antifungal Activity;    Candida Species;    Terbinafine;   

DOI  :  10.1186/1476-0711-9-7

 received in 2009-08-07, accepted in 2010-02-03,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundHydroxychavicol, isolated from the chloroform extraction of the aqueous leaf extract of Piper betle L., (Piperaceae) was investigated for its antifungal activity against 124 strains of selected fungi. The leaves of this plant have been long in use tropical countries for the preparation of traditional herbal remedies.MethodsThe minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of hydroxychavicol were determined by using broth microdilution method following CLSI guidelines. Time kill curve studies, post-antifungal effects and mutation prevention concentrations were determined against Candida species and Aspergillus species "respectively". Hydroxychavicol was also tested for its potential to inhibit and reduce the formation of Candida albicans biofilms. The membrane permeability was measured by the uptake of propidium iodide.ResultsHydroxychavicol exhibited inhibitory effect on fungal species of clinical significance, with the MICs ranging from 15.62 to 500 μg/ml for yeasts, 125 to 500 μg/ml for Aspergillus species, and 7.81 to 62.5 μg/ml for dermatophytes where as the MFCs were found to be similar or two fold greater than the MICs. There was concentration-dependent killing of Candida albicans and Candida glabrata up to 8 × MIC. Hydroxychavicol also exhibited an extended post antifungal effect of 6.25 to 8.70 h at 4 × MIC for Candida species and suppressed the emergence of mutants of the fungal species tested at 2 × to 8 × MIC concentration. Furthermore, it also inhibited the growth of biofilm generated by C. albicans and reduced the preformed biofilms. There was increased uptake of propidium iodide by C. albicans cells when exposed to hydroxychavicol thus indicating that the membrane disruption could be the probable mode of action of hydroxychavicol.ConclusionsThe antifungal activity exhibited by this compound warrants its use as an antifungal agent particularly for treating topical infections, as well as gargle mouthwash against oral Candida infections.

【 授权许可】

Unknown   
© Ali et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.