Free radical scavenging capacity, anticandicidal effect of bioactive compounds from Sida Cordifolia L., in combination with nystatin and clotrimazole and their effect on specific immune response in rats

Annals of Clinical Microbiology and Antimicrobials

Research

Alain Souza¹  Laya L Sawadogo²  Maurice Ouédraogo²  Kiessoun Konaté³  Alexis Nicaise Lepengué⁴  Bertrand M’Batchi⁵ 

[1] Laboratory of Animal Physiology, Electrophysiology and Pharmacology, Faculty of Science, University of Science and Technology of Masuku, P.O. Box: 943, Franceville, Gabon;Laboratory of Animal Physiology, University of Ouagadougou, 09 P.O. Box: 848, Ouagadougou, Burkina Faso;Laboratory of Biochemistry and Applied Chemistry, University of Ouagadougou, 09 P.O. Box: 848, Ouagadougou, Burkina Faso;Laboratory of Animal Physiology, Electrophysiology and Pharmacology, Faculty of Science, University of Science and Technology of Masuku, P.O. Box: 943, Franceville, Gabon;Laboratory of Plant Pathology, Faculty of Science, University of Science and Technology of Masuku, Franceville, P.O. Box: 943, Franceville, Gabon;Laboratory of Plant Pathology, Faculty of Science, University of Science and Technology of Masuku, Franceville, P.O. Box: 943, Franceville, Gabon;Laboratory of Animal Physiology, Electrophysiology and Pharmacology, Faculty of Science, University of Science and Technology of Masuku, P.O. Box: 943, Franceville, Gabon;

关键词: Minimum Inhibitory Concentration;    Antifungal Activity;    Clotrimazole;    Acute Toxicity Study;    Minimum Fungicidal Concentration;   

DOI  :  10.1186/1476-0711-11-33

 received in 2012-10-17, accepted in 2012-12-16,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundInfectious diseases caused by fungi are still a major threat to public health, despite numerous efforts by researchers. Use of ethnopharmacological knowledge is one attractive way to reduce empiricism and enhance the probability of success in new drug-finding efforts. In this work, the total alkaloid compounds (AC) from Sida cordifolia L. (Malvaceae) have been investigated for their free radical scavenging capacity, antifungal and immunostimulatory properties.MethodThe antifungal activity was investigated against five candida strains using the microplate dilution method and the Fractional Inhibitory Concentration Index (FICI) of compounds was evaluated. The antioxidant activity of the samples was evaluate using three separate methods, at last, the immunostimulatory effect on immunosuppressed wistar rats was performed.ResultsAs for the antifungal activity, result varied according to microorganism. The results obtained in this antifungal activity were interesting and indicated a synergistic effect between alkaloid compounds and the antifungal references such as Nystatin and Clotrimazole. Antioxidant capacity noticed that the reduction capacity of DPPH radicals obtained the best result comparatively to the others methods of free radical scavenging. Our results showed a low immunostimulatory effect and this result could be explained by the lack of biologically active antioxidants such as polyphenol compounds lowly contained in the alkaloid compounds.ConclusionThe results of this study showed that alkaloid compounds in combination with antifungal references (Nystatin and Clotrimazole) exhibited antimicrobial effects against candida strains tested. The results supported the utilization of these plants in infectious diseases particularly in treatment of candida infections.

【 授权许可】

CC BY   
© Ouédraogo et al.; licensee BioMed Central Ltd. 2012