Upregulation of NETO2 expression correlates with tumor progression and poor prognosis in colorectal carcinoma

BMC Cancer

Research Article

Chun-Fang Gao¹  Yan-Xia Zhai¹  Hao Fu¹  Chen-Ye Qi¹  Yu Zhang¹  Hui Gong¹  Liang Hu¹  Guang-Zhen Yang²  Jian Cai³  Qing-Ping Cai⁴  Hai-Yang Chen⁵  Dan Feng⁶ 

[1] Anal-Colorectal Surgery Institute, 150th Hospital of PLA, Luoyang, China;Department of Clinical Laboratory, 150th Hospital of PLA, Luoyang, China;Department of Colorectal Surgery, 150th Hospital of PLA, Luoyang, China;Department of Gastrointestine Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China;Department of Oncology, 150th Hospital of PLA, Luoyang, China;Department of Oncology, Changhai Hospital, Second Military Medical University, Shanghai, China;

关键词: NETO2;    Colorectal carcinoma;    Survival;    Prognosis;    Biomarker;   

DOI  :  10.1186/s12885-015-2018-y

 received in 2015-02-12, accepted in 2015-12-15,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundNeuropilin and tolloid-like 2 (NETO2) has been found to be overexpressed in different human cancers, but its expression pattern and clinical relevance in colorectal carcinoma (CRC) remains unknown.MethodsReal-time quantitative PCR, western blot and immunohistochemistry analyses were used to analyze the expression of NETO2 in CRC clinical samples. The correlation of NETO2 expression with clinicopathologic features was estimated in a cohort containing 292 patients with primary CRC. Kaplan-Meier and Cox proportional hazards regression analyses were used to assess the prognostic value of NETO2 expression in CRC.ResultsThe expression of NETO2 was frequently upregulated in CRC clinical samples at both the mRNA and protein levels, and its upregulation was significantly correlated with poor tumor differentiation (p = 0.013), advanced local invasion (p = 0.049), increased lymph node metastasis (p = 0.009), advanced TNM stage (p = 0.041) and increased patient death (p = 0.001). Kaplan-Meier analysis of the complete study cohort revealed that patients with high-NETO2 tumors had a significantly shorter disease-specific survival (DSS) than those with low-NETO2 tumors (p < 0.001). Importantly, high levels of NETO2 protein predicted poor DSS for patients with early stage tumors (p = 0.027) and for those with advanced stage tumors (p = 0.020). Furthermore, multivariate analyses indicated that increased NETO2 expression was an independent unfavorable prognostic factor for patients with early stage tumors (hazard ratio [HR] = 1.937, 95 % CI = 1.107-3.390, p = 0.021) as well as patients with advanced stage tumors (HR = 2.241, 95 % CI = 1.245-4.035, p = 0.007).ConclusionsOur findings suggest that NETO2 upregulation could serve as a potential biomarker for the prediction of advanced tumor progression and unfavorable prognosis in patients with CRC.

【 授权许可】

CC BY   
© Hu et al. 2015