BMC Genomics | |
No evidence for a bovine mastitis Escherichia coli pathotype | |
Research Article | |
Rolf Daniel1  Anja Poehlein1  Dennis Görlich2  Andreas Leimbach3  Ulrich Dobrindt4  John Vollmers5  | |
[1] Department of Genomic and Applied Microbiology, Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University of Göttingen, Göttingen, Germany;Institute of Biostatistics and Clinical Research, University of Münster, Münster, Germany;Institute of Hygiene, University of Münster, Mendelstrasse 7, 48149, Münster, Germany;Department of Genomic and Applied Microbiology, Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University of Göttingen, Göttingen, Germany;Institute for Molecular Infection Biology, Julius-Maximilians-University of Würzburg, Würzburg, Germany;Institute of Hygiene, University of Münster, Mendelstrasse 7, 48149, Münster, Germany;Institute for Molecular Infection Biology, Julius-Maximilians-University of Würzburg, Würzburg, Germany;Leibniz Institute DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany; | |
关键词: E. coli; Pathotype; Bovine mastitis; Commensals; Comparative genomics; Phylogeny; Virulence; Genomic diversity; | |
DOI : 10.1186/s12864-017-3739-x | |
received in 2016-12-27, accepted in 2017-04-27, 发布年份 2017 | |
来源: Springer | |
【 摘 要 】
BackgroundEscherichia coli bovine mastitis is a disease of significant economic importance in the dairy industry. Molecular characterization of mastitis-associated E. coli (MAEC) did not result in the identification of common traits. Nevertheless, a mammary pathogenic E. coli (MPEC) pathotype has been proposed suggesting virulence traits that differentiate MAEC from commensal E. coli. The present study was designed to investigate the MPEC pathotype hypothesis by comparing the genomes of MAEC and commensal bovine E. coli.ResultsWe sequenced the genomes of eight E. coli isolated from bovine mastitis cases and six fecal commensal isolates from udder-healthy cows. We analyzed the phylogenetic history of bovine E. coli genomes by supplementing this strain panel with eleven bovine-associated E. coli from public databases. The majority of the isolates originate from phylogroups A and B1, but neither MAEC nor commensal strains could be unambiguously distinguished by phylogenetic lineage. The gene content of both MAEC and commensal strains is highly diverse and dominated by their phylogenetic background. Although individual strains carry some typical E. coli virulence-associated genes, no traits important for pathogenicity could be specifically attributed to MAEC. Instead, both commensal strains and MAEC have very few gene families enriched in either pathotype. Only the aerobactin siderophore gene cluster was enriched in commensal E. coli within our strain panel.ConclusionsThis is the first characterization of a phylogenetically diverse strain panel including several MAEC and commensal isolates. With our comparative genomics approach we could not confirm previous studies that argue for a positive selection of specific traits enabling MAEC to elicit bovine mastitis. Instead, MAEC are facultative and opportunistic pathogens recruited from the highly diverse bovine gastrointestinal microbiota. Virulence-associated genes implicated in mastitis are a by-product of commensalism with the primary function to enhance fitness in the bovine gastrointestinal tract. Therefore, we put the definition of the MPEC pathotype into question and suggest to designate corresponding isolates as MAEC.
【 授权许可】
CC BY
© The Author(s). 2017
【 预 览 】
Files | Size | Format | View |
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RO202311107083958ZK.pdf | 1413KB | download |
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