Genome-wide analysis of the TPX2 family proteins in Eucalyptus grandis

BMC Genomics

Research Article

Yuqi Wang¹  Manoj Kumar²  Jinyan Wang³  Baolong Zhang³  Siming Gan⁴  Ai-Min Wu⁵  Qiaoli Xie⁵  Yuan Yao⁶  Pingzhou Du⁷ 

[1] College of Life Sciences, South China Agricultural University, 510642, Guangzhou, China;Boyce Thompson Institute for Plant Research, 14853, Ithaca, USA;Faculty of Life Science, University of Manchester, Michael Smith Building, Oxford Road, M13 9PT, Manchester, UK;Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, China;Research Institute of tropical forestry, Chinese Academy of Forestry, 510520, Guangzhou, China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, 510642, Guangzhou, China;Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, 510642, Guangzhou, China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, 510642, Guangzhou, China;Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, 510642, Guangzhou, China;College of Life Sciences, South China Agricultural University, 510642, Guangzhou, China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, 510642, Guangzhou, China;Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, 510642, Guangzhou, China;College of Life Sciences, South China Agricultural University, 510642, Guangzhou, China;Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, China;

关键词: Eucalyptus;    TPX2 family proteins;    Phylogenetic analysis;    Expression profiling;    Subcellular localization;    Organ twisting;   

DOI  :  10.1186/s12864-016-3303-0

 received in 2015-12-11, accepted in 2016-11-16,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe Xklp2 (TPX2) proteins belong to the microtubule-associated (MAP) family of proteins. All members of the family contain the conserved TPX2 motif, which can interact with microtubules, regulate microtubule dynamics or assist with different microtubule functions, for example, maintenance of cell morphology or regulation of cell growth and development. However, the role of members of the TPX family have not been studied in the model tree species Eucalyptus to date. Here, we report the identification of the members of the TPX2 family in Eucalyptus grandis (Eg) and analyse the expression patterns and functions of these genes.ResultsIn present study, a comprehensive analysis of the plant TPX2 family proteins was performed. Phylogenetic analyses indicated that the genes can be classified into 6 distinct subfamilies. A genome-wide survey identified 12 members of the TPX2 family in the sequenced genome of Eucalyptus grandis. The basic genetic properties of the TPX2 family in Eucalyptus were analysed. Our results suggest that the TPX2 family proteins within different sub-groups are relatively conserved but there are important differences between groups. Quantitative real-time PCR (qRT-PCR) was performed to confirm the expression levels of the genes in different tissues. The results showed that in the whole plant, the levels of EgWDL5 transcript are the highest, followed by those of EgWDL4. Compared with other tissues, the level of the EgMAP20 transcript is the highest in the root. Over-expression of EgMAP20 in Arabidopsis resulted in organ twisting. The cotyledon petioles showed left-handed twisting while the hypocotyl epidermal cells produced right-handed helical twisting. Finally, EgMAP20, EgWDL3 and EgWDL3L were all able to decorate microtubules.ConclusionsPlant TPX2 family proteins were systematically analysed using bioinformatics methods. There are 12 TPX2 family proteins in Eucalyptus. We have performed an initial characterization of the functions of several members of the TPX2 family. We found that the gene products are localized to the microtubule cytoskeleton. Our results lay the foundation for future efforts to reveal the biological significance of TPX2 family proteins in Eucalyptus.

【 授权许可】

CC BY   
© The Author(s). 2016