Severe teratozoospermia and its influence on pronuclear morphology, embryonic cleavage and compaction

Reproductive Biology and Endocrinology

Research

James Goldfarb¹  Nina Desai¹  Faten AbdelHafez¹  Dara S Berger²  Helena Russell³ 

[1] Department of OB-GYN, Cleveland Clinic Foundation, 26900 Cedar Road, 44122, Cleveland, Ohio, USA;Department of OB-GYN, Cleveland Clinic Foundation, 26900 Cedar Road, 44122, Cleveland, Ohio, USA;Department of OB-GYN, Eastern Virginia Medical School, School of Health Professions, 601 Colley Avenue, 23507, Norfolk, Virginia, USA;Department of OB-GYN, Eastern Virginia Medical School, School of Health Professions, 601 Colley Avenue, 23507, Norfolk, Virginia, USA;

关键词: Clinical Pregnancy;    Clinical Pregnancy Rate;    Sperm Morphology;    ICSI Cycle;    Paternal Effect;   

DOI  :  10.1186/1477-7827-9-37

 received in 2010-11-08, accepted in 2011-03-22,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundFertilization, cell division and embryo development depend on genomic contributions from male and female gametes. We hypothesize that teratozoospermic sperm influences early embryo development and embryo compaction.MethodsWe conducted a retrospective analysis of embryos derived from intracytoplasmic sperm injection (ICSI) cycles. Two hundred thirty-five consecutive ICSI cycles were included in the study; all treatment was provided at the Cleveland Clinic Fertility Center. Patient cycles were divided by sperm morphology based on Kruger's strict criteria: Group A, embryos where teratozoospermic sperm (0-2% normal) were used for ICSI and Group B, embryos where dysmorphic sperm (5-13% normal) were used for ICSI. All cycles analyzed were of patients doing day 3 embryo transfers. Outcome measures assessed included pronuclear (PN) pattern, syngamy, early cleavage, cell number, rate of compaction and blastulation of embryos left in culture and not transferred on day 3.ResultsA total of 1762 embryos were analyzed. PN patterns were similar in Group A and Group B embryos. No differences were noted in syngamy, cleavage, cell number or blastulation rate. Studying the development of embryos in culture after day 3 transfer revealed a difference in the timeline for compaction. By day 4, 25% of Group A embryos had compacted compared to 36% in Group B (P = 0.0007). There was no difference found between Group A and Group B embryos in regards to blastulation.ConclusionsWe did not find an association between sperm morphology and clinical outcomes. The impact of teratozoospermia may be masked in ICSI cycles where fertilization, implantation rate and clinical pregnancy rate are the primary outcome measures. However, by examining the timeline of development, we were better able to discern a potential paternal effect at critical transition points from fertilization through activation.

【 授权许可】

Unknown   
© Berger et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.