miR-183 inhibits TGF-β1-induced apoptosis by downregulation of PDCD4 expression in human hepatocellular carcinoma cells

BMC Cancer

Research Article

Hanjiang Fu¹  Zhixian Sun¹  Ruiyun Xing¹  Xiaofei Zheng¹  Yi Tie¹  Chengwang Xu¹  Jie Zhu¹  Jipeng Li²  Yide Qin³ 

[1] Beijing Institute of Radiation Medicine, 27 Taiping Road, 100850, Beijing, P.R. China;Beijing Institute of Radiation Medicine, 27 Taiping Road, 100850, Beijing, P.R. China;Department of Biochemistry and Molecular Biology, Anhui Medical University, 81 Meishan Road, 230032, Hefei, P.R. China;Department of Biochemistry and Molecular Biology, Anhui Medical University, 81 Meishan Road, 230032, Hefei, P.R. China;

关键词: HepG2 Cell;    Huh7 Cell;    PDCD4 Expression;    PDCD4 Protein;    Adjacent Nontumoral Liver;   

DOI  :  10.1186/1471-2407-10-354

 received in 2009-10-26, accepted in 2010-07-06,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundIn recent years, some miRNAs have been reported to be connected closely with the development of human hepatocellular carcinoma. In our previous studies, a set of miRNAs were revealed to be dysregulated in HCC tissues. However, the functions of these miRNAs in HCC remain largely undefined.MethodsThe expression profiles of miR-183 were compared between HCC tissues and adjacent normal liver tissues using qRT-PCR method. This method was used to screen the potential target genes of miR-183. A luciferase reporter assay was conducted to confirm target association. Finally, the functional effect of miR-183 in hepatoma cells was examined.ResultsAmong the 25 HCC samples analyzed, microRNA-183 was significantly up-regulated (twofold to 367-fold) in 17 samples compared with the matching nontumoral liver tissues. Programmed cell death 4 (PDCD4) was identified as the target gene of miR-183. Moreover, PDCD4 is a proapoptotic molecule involved in TGF-β1-induced apoptosis in human HCC cells, we found that miR-183 transfectants were resistant to apoptosis induced by TGF-β1.ConclusionsWe conclude that miR-183 can inhibit apoptosis in human HCC cells by repressing the PDCD4 expression, and miR-183 may play an important role in HCC development.

【 授权许可】

Unknown   
© Li et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.