Small nucleolar RNA 113–1 suppresses tumorigenesis in hepatocellular carcinoma

Molecular Cancer

Research

Fang Yang¹  Ping Zhao²  Zhong-Tian Qi²  Wen Wang²  Cui-Ling Ding²  Lan-Juan Zhao²  Hao Ren²  Gang Xu² 

[1] Department of Hepatobiliary Surgery, Fuzhou General Hospital of Nanjing Military Area Command, 350025, Fuzhou, China;Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, 800 XiangYin RD, 200433, Shanghai, China;

关键词: HepG2 Cell;    Huh7 Cell;    Putative Promoter Region;    Differentially Methylated Region;    snoRNA Gene;   

DOI  :  10.1186/1476-4598-13-216

 received in 2014-05-04, accepted in 2014-09-09,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundEmerging evidence suggests that small nucleolar RNAs (snoRNAs) are involved in tumorigenesis. The roles of small nucleolar RNA 113–1 (SNORD113-1) on the development of hepatocellular carcinoma (HCC) remain unknown.MethodsThe expression of SNORD113-1 was measured in 112 HCC tumor tissues using quantitative RT-PCR and compared with expression levels from with paired non-tumor tissues. The effects of SNORD113-1 on HCC tumorigenesis were investigated in HepG2 and Huh7 cells as well as a xenograft nude mouse model. CpG methylation within the promoter region of the SNORD113-1 gene was identified using Sodium bisulfite sequencing. Cancer pathway reporter investigate the mechanism by which SNORD113-1 suppressed tumorigenesis.ResultsSNORD113-1 expression was significantly downregulated in HCC tumors compared with adjacent non-tumor tissues, and downregulation of SNORD113-1 in HCC tumors was significantly associated with worse survival of patients. In addition, CpG methylation at the promoter region of the SNORD113-1 gene was higher in HCC tumors than adjacent non-tumor tissues. Functionally, SNORD113-1 suppressed cancer cell growth in HepG2 and Huh7 cells and in a xenograft nude mouse model. Furthermore, SNORD113-1 inactivated the phosphorylation of ERK1/2 and SMAD2/3 in MAPK/ERK and TGF-β pathways.ConclusionsSNORD113-1 functions as a tumor suppressor role in HCC and may be important as a potential diagnostic and therapeutic target for HCC.

【 授权许可】

Unknown   
© Xu et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.