Effect of Spp1 on nerve degeneration and regeneration after rat sciatic nerve injury

BMC Neuroscience

Research Article

Xingyu Liu¹  Shusen Cui¹  Dengbing Yao²  Yuhua Sun²  Yuting Li²  Meiyuan Li²  Huaiqin Li²  Ying Yuan³ 

[1] China-Japan Union Hospital of Jilin University, 126 Xiantai Road, 130033, Changchun, Jilin, People’s Republic of China;School of Life Sciences, Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, 19 Qixiu Road, 226001, Nnatong, Jiangsu, People’s Republic of China;School of Life Sciences, Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, 19 Qixiu Road, 226001, Nnatong, Jiangsu, People’s Republic of China;Affiliated Hospital of Nantong University, 20 Xisi Road, 226001, Nantong, Jiangsu, People’s Republic of China;

关键词: Wallerian degeneration;    Secreted phosphoprotein 1 (Spp1);    Schwann cells;    Nerve regeneration;    Rat;    Sciatic nerve injury;   

DOI  :  10.1186/s12868-017-0348-1

 received in 2016-11-08, accepted in 2017-02-24,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundWallerian degeneration (WD) in injured peripheral nerves is associated with a large number of up- or down-regulated genes, but the effects of these changes are poorly understood. In our previous studies, we reported some key factors that are differentially expressed to activate nerve degeneration and regeneration during WD. Here, we determined the effects of secreted phosphoprotein 1 (Spp1) on WD after rat sciatic nerve injury.ResultsSpp1 was upregulated from 6 h to 14 days after sciatic nerve injury. Altered expression of Spp1 in Schwann cells (SC) resulted in altered mRNA and protein expression levels for cytokines, c-Fos, PKCα and phospho-ERK/ERK and affected SC apoptosis in vitro. Silencing of Spp1 expression in SCs using siRNA technology reduced proliferation and promoted migration of SCs in vitro. By contrast, overexpression of Spp1 promoted proliferation and reduced migration in SCs in vitro. Differential expression of Spp1 after sciatic nerve injury in vivo altered the expression of cytokines, c-Fos, PKCα, and the p-ERK/ERK pathway.ConclusionsSpp1 is a key regulatory factor that affects nerve degeneration and regeneration through c-Fos, PKCα and p-ERK/ERK pathways after rat sciatic nerve injury. These results shed new light on the role of Spp1 in nerve degeneration and regeneration during WD.

【 授权许可】

CC BY   
© The Author(s) 2017